Kang Ning, Li Fu-cai, Fu Wei-neng, Zhang Jing-hai, Sun Kai-lai
Department of Medical Genetics, College of Basic Medical Sciences, China Medical Univercity, Shenyang, Liaoning, 110001, PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Apr;24(2):131-5.
To search for characteristic chromosome changes in primary laryngeal squamous cell carcinoma (LSCC) and Hep-2 cell line and to realize the relationship between the cytogenetic abnormality and the pathogenetic mechanism in LSCC.
The fresh resulted samples of LSCC were analyzed with an improved primary cell culture for chromosome preparation and G-banding technique. Hep-2 cell line was analyzed by high resolution banding technique. Molecular cytogenetics analysis was made by chromosome 6 painting probe.
Four primary LSCC succeeded in primary cell culture and obtained metaphases, one was tetraploid, the other three were triploid. The chromosome mode of Hep-2 cell line was from 68 to 75 and fifteen marker chromosomes were found. The most structural abnormalities of chromosome in primary LSCC and HEP-2 cell line were unbalance translocation, terminal deletion and isochromosome. The complicate aberration in chromosome 6 was common in LSCC and Hep-2.
6q-, I(5p), 17p-, 5q- are considered as characteristic chomosome changs in LSCC. Fluorescence in situ hybridization (FISH) may enhance the ability of detecting complicated chromosome rearrangements and marker chromosomes, which could provide more value data to verify the chromosome characteristic aberration in LSCC.
探寻原发性喉鳞状细胞癌（LSCC）及Hep-2细胞系的特征性染色体变化，以明确LSCC细胞遗传学异常与发病机制之间的关系。
采用改良的原代细胞培养法制备染色体并运用G显带技术，对新鲜的LSCC标本进行分析。采用高分辨显带技术分析Hep-2细胞系。运用6号染色体涂染探针进行分子细胞遗传学分析。
4例原发性LSCC成功进行原代细胞培养并获得中期分裂相，1例为四倍体，另3例为三倍体。Hep-2细胞系的染色体众数为68至75，发现15条标记染色体。原发性LSCC及Hep-2细胞系中染色体最常见的结构异常为不平衡易位、末端缺失及等臂染色体。6号染色体的复杂畸变在LSCC及Hep-2中较为常见。
6q-、I(5p)、17p-、5q-被认为是LSCC的特征性染色体变化。荧光原位杂交（FISH）可增强检测复杂染色体重排及标记染色体的能力，能为验证LSCC染色体特征性畸变提供更有价值的数据。
