[应用荧光原位杂交技术检测Hep-2细胞及肝癌细胞系染色体畸变]
[Detection of Hep-2 and hepatoma cell line chromosomal aberration by using fluorescence in situ hybridization].
作者信息
Pan S, Zhang X, Wang F, Zhao W, Li C, Chen Y
机构信息
Department of Biology, Najing Normal University, Nanjing, Jiangsu, 210097 P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Oct 10;15(5):278-80.
OBJECTIVE
To study the chromosomal aberration of hepatoma cell line (1172) and laryngocarcinoma cell line (Hep-2).
METHODS
Fluorescence in situ hybridization (FISH) was used with nine chromosomal special libraries.
RESULTS
The abnormal signals indicated that very complicated aberrations existed in the two cell lines. This suggested that the aberrations also existed in other chromosomes not yet studied. In 1172, the major type was structure aberration, but in Hep-2, numerical abnormality was shown in almost all chromosomes studied.
CONCLUSION
Compared with traditional cytological methods, this technique has the advantages of being quicker and more accurate and sensitive.
目的
研究肝癌细胞系（1172）和喉癌细胞系（Hep-2）的染色体畸变情况。
方法
使用九个染色体特殊文库进行荧光原位杂交（FISH）。
结果
异常信号表明这两种细胞系中存在非常复杂的畸变。这表明在尚未研究的其他染色体中也存在畸变。在1172细胞系中，主要类型是结构畸变，但在Hep-2细胞系中，几乎所有研究的染色体都显示出数目异常。
结论
与传统细胞学方法相比，该技术具有更快、更准确和更灵敏的优点。
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