Butkevich I P, Barr G A, Vershinina E A
Zh Evol Biokhim Fiziol. 2007 Jan-Feb;43(1):54-9.
In experiments on the 7-day-old female and male Long-Evans rat pups, for the first time, there was studied effect of prenatal (immobilization) stress on dynamics of nociceptive behavioral response caused by an inflammatory focus. The nociceptive sensitivity was evaluated for 1 h by the number of 7-day-old organized at the spinal level in response to injection of formalin (10%, 10 microl) to the posterior leg sole. Control rat pups were not submitted to any prenatal stress; in these animals the response in the formalin test was found to be represented by one phase. It the prenatally stressed rat pups the studied patterns were organized into two phases characteristic of the definitive type of response. At the period between them (during interphase), the nociceptive behavior was absent. At the second, tonic phase the number of flexes+shakes in the prenatally stressed males was statistically significantly higher than in the prenatally stressed females, which indicates a sensitization of the neurons involved in the tonic pain chains in male individuals. Thus, the data obtained on prenatally stressed animals confirm the previous data about immaturity of the mechanisms mediating the second phase of response in the formalin test in the 7-day-old rat pups. An important fact is revealed which indicates that in the prenatally stressed rat pups of the same age the second phase of response is already obvious. Mechanisms underlying the behavioral response caused by the inflammatory focus in the formalin test in the number flexes + shakes old stressed rat pups are characterized by sexual dimorphism: the pain sensitivity in males at the second phase of response is statistically significantly higher than in females.
在对7日龄雌性和雄性Long-Evans幼鼠进行的实验中，首次研究了产前（固定）应激对由炎症病灶引起的伤害性行为反应动态的影响。通过对后肢足底注射福尔马林（10%，10微升）后，7日龄幼鼠在脊髓水平上组织的反应数量来评估伤害性敏感性，持续1小时。对照幼鼠未遭受任何产前应激；在这些动物中，福尔马林试验中的反应表现为一个阶段。在产前应激的幼鼠中，所研究的模式被组织成确定反应类型的两个阶段。在这两个阶段之间的时期（间期），没有伤害性行为。在第二个紧张期，产前应激雄性幼鼠的屈曲+抖动次数在统计学上显著高于产前应激雌性幼鼠，这表明雄性个体中参与紧张性疼痛链的神经元发生了敏化。因此，在产前应激动物身上获得的数据证实了先前关于7日龄幼鼠福尔马林试验中介导第二阶段反应的机制不成熟的数据。揭示了一个重要事实，即相同年龄的产前应激幼鼠中，反应的第二阶段已经很明显。福尔马林试验中炎症病灶引起的行为反应的潜在机制，以7日龄应激幼鼠的屈曲+抖动次数为特征，具有性别二态性：反应第二阶段雄性的疼痛敏感性在统计学上显著高于雌性。
