Mikhailenko V A, Butkevich I P, Vershinina E A, Semenov P O
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, 6 Makarov Bank, 199034 St. Petersburg, Russia.
Neurosci Behav Physiol. 2010 Feb;40(2):179-84. doi: 10.1007/s11055-009-9241-4. Epub 2009 Dec 22.
The interrelationship between measures of pain reactions (number of flexion + shaking patterns) in the formalin test and the level of depression (duration of immobility) in the forced swimming (Porsolt) test was studied in prenatally stressed rat pups aged 7-8 days. Two series of experiments were performed, with different sequences of tests separated by intervals of one day. In the first series of experiments, the Porsolt test was performed first; in the second series, the formalin test was performed before forced swimming. The sequence of tests was found to have different effects on measures of pain and depression and their correlation in prenatally stressed and unstressed rat pups. The effects of the sequence of the depression test (before or after the formalin test) on measures of depression were different in prenatally unstressed and stressed rat pups. In the former there were no differences between the two test sequences, while in prenatally stressed rat pups the first sequence showed a significant increase in the duration of immobility. The order of testing had no effect on the pain response--there were no differences between the numbers of flexion + shaking patterns in either prenatally stressed rat pups or unstressed animals; measures of the pain response were significantly greater in the sequence in which the formalin test was followed by the Porsolt test in prenatally stressed individuals as compared with unstressed animals. A positive correlation between study parameters was seen in the first series in prenatally unstressed rat pups, while there was a negative correlation in prenatally stressed animals. In the second series, there were no significant relationships between measures. Thus, the sequelae of postnatal stress, as imposed by each test the day before the final test, were apparent only in prenatally stressed animals in terms of the level of depression.
在7 - 8日龄的产前应激大鼠幼崽中，研究了福尔马林试验中疼痛反应指标（屈曲 + 颤抖模式的数量）与强迫游泳（波索尔特）试验中抑郁水平（不动持续时间）之间的相互关系。进行了两个系列的实验，不同的测试顺序间隔一天。在第一个系列实验中，先进行波索尔特试验；在第二个系列中，在强迫游泳前进行福尔马林试验。发现测试顺序对产前应激和未应激大鼠幼崽的疼痛和抑郁指标及其相关性有不同影响。抑郁测试顺序（福尔马林试验之前或之后）对抑郁指标的影响在产前未应激和应激大鼠幼崽中有所不同。在前者中，两种测试顺序之间没有差异，而在产前应激大鼠幼崽中，第一种顺序显示不动持续时间显著增加。测试顺序对疼痛反应没有影响——产前应激大鼠幼崽或未应激动物的屈曲 + 颤抖模式数量之间没有差异；与未应激动物相比，在产前应激个体中，福尔马林试验后进行波索尔特试验的顺序中疼痛反应指标显著更高。在第一个系列中，产前未应激大鼠幼崽的研究参数之间呈正相关，而在产前应激动物中呈负相关。在第二个系列中，各指标之间没有显著关系。因此，就抑郁水平而言，在最终测试前一天每项测试所施加的产后应激后遗症仅在产前应激动物中明显。
