Butkevich Irina P, Mikhailenko Victor A, Vershinina Elena A, Semionov Pavel O, Otellin Vladimir A, Aloisi Anna Maria
Laboratory of Ontogeny of the Nervous System, Pavlov Institute of Physiology, Russian Academy of Sciences, Nab. Makarova, 6, St. Petersburg 199034, Russia.
Brain Res. 2009 Aug 25;1286:53-9. doi: 10.1016/j.brainres.2009.06.055. Epub 2009 Jun 25.
The infant stage of rat development is a very important period for potential correction of adverse consequences produced by negative prenatal events. However the age limit for this correction needs to be investigated. The last prenatal and first two weeks after birth are "critical" for maturation of the nociceptive and emotional systems. Clinical observations suggest a correlation between persistent pain response and emotional behavior. In infant male rats of different ages, we studied indices of the inflammatory pain response (the number of flexes+shakes in the formalin test), depression-related behavior (immobility in the forced swim test) and the relations between them, as well as the effects of prenatal stress on these indices. Furthermore, we assessed the trend of body weight and the relations between body weight and the depression- and pain-related behaviors. We demonstrate heterogeneity of the infant stage: control prenatally non-stressed rat pups showed significantly lower immobility at 7 days of age than at 10 days; prenatal stress caused an increase of immobility and the number of flexes+shakes in 7-8-day-old pups but not in 10-11-day-olds. These findings should be taken into account in the treatment of abnormalities of emotional and inflammatory pain-related behaviors produced by prenatal stressful events. The present data and our previous findings indicate that the deficiency of body weight in prenatally stressed newborns may predict the development of abnormalities in inflammatory pain-related responses during postnatal ontogeny.
大鼠发育的婴儿期是潜在纠正产前负面事件所产生不良后果的非常重要的时期。然而，这种纠正的年龄限制需要进行研究。产前的最后阶段以及出生后的头两周对于伤害感受和情绪系统的成熟至关重要。临床观察表明持续疼痛反应与情绪行为之间存在关联。在不同年龄的雄性幼鼠中，我们研究了炎症性疼痛反应指标（福尔马林试验中的屈曲+抖动次数）、抑郁相关行为（强迫游泳试验中的不动时间）以及它们之间的关系，以及产前应激对这些指标的影响。此外，我们评估了体重变化趋势以及体重与抑郁和疼痛相关行为之间的关系。我们证明了婴儿期的异质性：产前未受应激的对照幼鼠在7日龄时的不动时间显著低于10日龄时；产前应激导致7-8日龄幼鼠的不动时间和屈曲+抖动次数增加，但10-11日龄幼鼠未出现这种情况。在治疗产前应激事件所产生的情绪和炎症性疼痛相关行为异常时应考虑这些发现。目前的数据以及我们之前的研究结果表明，产前受应激新生儿的体重不足可能预示着出生后个体发育过程中炎症性疼痛相关反应异常的发生。
