Goto Yasuyuki, Sanjoba Chizu, Arakaki Nana, Okamoto Masaaki, Saeki Keiichi, Onodera Takashi, Ito Mamoru, Matsumoto Yoshitsugu
Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Parasitol Int. 2007 Sep;56(3):231-4. doi: 10.1016/j.parint.2007.02.007. Epub 2007 Mar 12.
Migration inhibitory factor-related protein 8 (MRP8) and MRP14 are expressed by myeloid cells and especially known as marker proteins of an immature and inflammatory subtype of macrophages. In this study, we immunohistochemically examined an accumulation of MRP8+ and MRP14+ macrophages in skin lesions during Leishmania major infection in susceptible BALB/c and RAG-2-/- mice. L. major infection caused the development of a nodular type of skin lesion at the infection site in mice and a massive accumulation of macrophages was observed in the lesions at four weeks after the infection. Immunohistochemical analyses showed MRP8+ and MRP14+ macrophages are predominant cell types in the skin lesions in both mouse strains. In contrast, F4/80+ cells, which correspond to mature macrophages, were rarely found in the skin lesions. These data suggest that the accumulation of inflammatory subtype of macrophages in BALB/c mice during L. major infection can be induced without acquired immune responses.
迁移抑制因子相关蛋白8（MRP8）和MRP14由髓样细胞表达，尤其被认为是未成熟和炎症亚型巨噬细胞的标志物蛋白。在本研究中，我们采用免疫组织化学方法检测了易感的BALB/c和RAG-2-/-小鼠在感染硕大利什曼原虫期间皮肤病变中MRP8+和MRP14+巨噬细胞的聚集情况。硕大利什曼原虫感染导致小鼠感染部位出现结节型皮肤病变，感染四周后在病变部位观察到大量巨噬细胞聚集。免疫组织化学分析表明，MRP8+和MRP14+巨噬细胞是两种小鼠品系皮肤病变中的主要细胞类型。相比之下，对应于成熟巨噬细胞的F4/80+细胞在皮肤病变中很少发现。这些数据表明，在硕大利什曼原虫感染期间，BALB/c小鼠中炎症亚型巨噬细胞的聚集可以在没有获得性免疫反应的情况下被诱导。
