Nagai Kazuki, Fujii Wataru, Yamagishi Junya, Sanjoba Chizu, Goto Yasuyuki
Laboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Laboratory of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Pathogens. 2024 Mar 6;13(3):232. doi: 10.3390/pathogens13030232.
Visceral leishmaniasis (VL) is an infectious disease caused by parasitic protozoa of the genus and manifests clinical symptoms such as splenomegaly, hepatomegaly, anemia, and fever. It has previously been shown that B-cell-activating factor (BAFF) is involved in splenomegaly during VL. Although BAFF is known to be expressed by a variety of cells, the mechanism of elevated BAFF expression in VL is not clear. In this study, we aimed to identify BAFF-producing cells in the spleens of mice infected with . Splenocytes of . -infected mice showed elevated BAFF expression compared to that of naive mice. In the infected spleen, the number of both CD11b and F4/80 cells increased, and the major BAFF-producing cells were CD11b cells, which did not serve as host cells of . Immunohistochemical/immunofluorescent staining of spleens of infected mice revealed that the increased CD11b cells were primarily MRP14 mononuclear cells. Together, these results suggest the increased BAFF expression in the spleen of . -infected mice involves a recruitment of inflammatory macrophages distinct from host macrophages for the parasites.
内脏利什曼病（VL）是由利什曼原虫属的寄生原生动物引起的一种传染病，表现出脾肿大、肝肿大、贫血和发热等临床症状。先前的研究表明，B细胞活化因子（BAFF）与VL期间的脾肿大有关。尽管已知BAFF由多种细胞表达，但VL中BAFF表达升高的机制尚不清楚。在本研究中，我们旨在鉴定感染利什曼原虫的小鼠脾脏中产生BAFF的细胞。与未感染的小鼠相比，感染利什曼原虫的小鼠脾细胞显示出BAFF表达升高。在受感染的脾脏中，CD11b细胞和F4/80细胞的数量均增加，主要产生BAFF的细胞是CD11b细胞，而CD11b细胞不是利什曼原虫的宿主细胞。对感染小鼠脾脏进行免疫组织化学/免疫荧光染色显示，增加的CD11b细胞主要是MRP14单核细胞。总之，这些结果表明，感染利什曼原虫的小鼠脾脏中BAFF表达增加涉及募集与寄生虫宿主巨噬细胞不同的炎性巨噬细胞。
