Empson Ruth M, Garside Molly L, Knöpfel Thomas
School of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, United Kingdom.
J Neurosci. 2007 Apr 4;27(14):3753-8. doi: 10.1523/JNEUROSCI.0069-07.2007.
Plasma membrane Ca2+ ATPase 2 (PMCA2) is a fast, highly effective mechanism to control resting cytosolic Ca2+ and Ca2+ excursions in neurons and other excitable cells. The strong expression of PMCA2 in the cerebellum and the cerebellar behavioral deficits presented by PMCA2-/- knock-out mice all point to its importance for cerebellar circuit dynamics. Here, we provide direct functional evidence for the influence of presynaptic PMCA2-mediated Ca2+ extrusion for short-term plasticity at cerebellar parallel fiber to Purkinje neuron synapses. Dramatic structural alterations to the Purkinje neurons in the absence of PMCA2 also suggest a strong influence of this fast PMCA2 isoform for development and maintenance of cerebellar function.
质膜Ca2+ ATP酶2（PMCA2）是一种快速、高效的机制，用于控制神经元和其他可兴奋细胞中的静息胞质Ca2+及Ca2+波动。PMCA2在小脑中的强表达以及PMCA2基因敲除小鼠出现的小脑行为缺陷均表明其对小脑回路动力学的重要性。在此，我们提供了直接的功能证据，证明突触前PMCA2介导的Ca2+外排在小脑平行纤维至浦肯野神经元突触的短期可塑性方面的影响。在缺乏PMCA2的情况下，浦肯野神经元发生的显著结构改变也表明这种快速的PMCA2亚型对小脑功能的发育和维持有强烈影响。
