Gargaglioni Luciane H, Meier Janice T, Branco Luiz G S, Milsom William K
Departamento de Morfologia e Fisiologia Animal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista Júlio de Mesquita Filho, Via de acesso Paulo Donato Castellane s/n, 14870-000 Jaboticabal, SP, Brazil.
Am J Physiol Regul Integr Comp Physiol. 2007 Jul;293(1):R447-57. doi: 10.1152/ajpregu.00793.2006. Epub 2007 Apr 4.
The present study was designed to explore systematically the midbrain of unanesthetized, decerebrate anuran amphibians (bullfrogs), using chemical and electrical stimulation and midbrain transections to identify sites capable of exciting and inhibiting breathing. Ventilation was measured as fictive motor output from the mandibular branch of the trigeminal nerve and the laryngeal branch of the vagus nerve. The results of our transection studies suggest that, under resting conditions, the net effect of inputs from sites within the rostral half of the midbrain is to increase fictive breathing frequency, whereas inputs from sites within the caudal half of the midbrain have no net effect on fictive breathing frequency but appear to act on the medullary central rhythm generator to produce episodic breathing. The results of our stimulation experiments indicate that the principal sites in the midbrain that are capable of exciting or inhibiting the fictive frequency of lung ventilation, and potentially clustering breaths into episodes, appear to be those primarily involved in visual and auditory integration, motor functions, and attentional state.
本研究旨在系统地探索未麻醉、去脑的无尾两栖动物（牛蛙）的中脑，采用化学和电刺激以及中脑横切术来确定能够兴奋和抑制呼吸的部位。通过测量来自三叉神经下颌支和迷走神经喉支的虚拟运动输出作为通气情况。我们的横切研究结果表明，在静息状态下，来自中脑 Rostral 半部分内各部位的输入的净效应是增加虚拟呼吸频率，而来自中脑 Caudal 半部分内各部位的输入对虚拟呼吸频率没有净效应，但似乎作用于延髓中央节律发生器以产生间歇性呼吸。我们的刺激实验结果表明，中脑中能够兴奋或抑制肺通气虚拟频率，并可能将呼吸聚集成发作的主要部位，似乎是那些主要参与视觉和听觉整合、运动功能以及注意力状态的部位。
