Nishimura Masuhiro, Naito Shinsaku
Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc, Japan.
Biol Pharm Bull. 2007 Apr;30(4):821-5. doi: 10.1248/bpb.30.821.
Pairs of forward and reverse primers and TaqMan probes specific to each of 15 human sulfotransferases were prepared. The mRNA expression level of each target enzyme was analyzed in total RNA from single and pooled specimens of various human tissues (adrenal gland, bone marrow, brain, colon, heart, kidney, liver, lung, pancreas, peripheral leukocytes, placenta, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testis, thymus, thyroid gland, trachea, and uterus) by real-time reverse transcription PCR using an ABI PRISM 7700 Sequence Detection System. The mRNA expression profiles of the sulfotransferases in these 23 different human tissues were used to identify the tissues exhibiting high transcriptional activity for these enzymes. These results provide valuable information for studies concerning the human carbohydrate sulfotransferase and tyrosylprotein sulfotransferase genes in various tissues.
制备了针对15种人类磺基转移酶的正向和反向引物对以及TaqMan探针。使用ABI PRISM 7700序列检测系统，通过实时逆转录PCR分析了来自各种人类组织（肾上腺、骨髓、脑、结肠、心脏、肾脏、肝脏、肺、胰腺、外周血白细胞、胎盘、前列腺、唾液腺、骨骼肌、小肠、脊髓、脾脏、胃、睾丸、胸腺、甲状腺、气管和子宫）的单个和混合样本的总RNA中每种靶酶的mRNA表达水平。利用这23种不同人类组织中磺基转移酶的mRNA表达谱来鉴定对这些酶表现出高转录活性的组织。这些结果为研究各种组织中的人类碳水化合物磺基转移酶和酪氨酰蛋白磺基转移酶基因提供了有价值的信息。
