Lee Byung Joo, Wang Soo Geun, Lee Jin Choon, Jung Jin Sup, Bae Yong Chan, Jeong Hae Jin, Kim Hwal Woong, Lorenz Robert R
Department of Otolaryngology, College of Medicine, and Medical Research Institute, Pusan National University, Pusan, Korea.
Cells Tissues Organs. 2006;184(3-4):198-204. doi: 10.1159/000099627.
Prevention and treatment of vocal fold scarring and atrophy remain challenging. The aim of this study was to treat injured vocal folds using autologous adipose tissue-derived stromal cells (ADSCs) and evaluate the ability to prevent vocal fold scarring and atrophy by ADSCs in a canine animal model. Ten adult dogs were used for this experiment. ADSCs from the adipose tissue from the inguinal area were isolated and cultured in all dogs. Immediately after being mixed with atelocollagen, the ADSCs (1-3 x 10(6)) were injected into the right vocal fold of each animal, using a syringe with a 23-gauge needle. As a control, atelocollagen was injected into the left vocal fold of the same dog. The effects of the prevention of vocal fold scarring and atrophy were measured by morphological and histological assessment. At 8 weeks, there was a difference in granuloma and atrophic changes between the ADSC-injected and control sides in the majority of the dogs. This difference continued to be present at the 24 weeks' follow-up. On histopathologic examination, a large number of cells labeled with a fluorochrome were observed in ADSC-injected vocal folds 8 weeks after the initial treatment. This study demonstrates the multipotential ability of ADSCs in the regeneration of injured vocal folds. Injecting ADSCs into a damaged vocal fold appears to be useful in preventing vocal fold scarring and atrophy 24 weeks after initial damage.
声带瘢痕形成和萎缩的预防与治疗仍然具有挑战性。本研究的目的是使用自体脂肪组织来源的基质细胞（ADSCs）治疗受损声带，并在犬类动物模型中评估ADSCs预防声带瘢痕形成和萎缩的能力。十只成年犬用于本实验。所有犬均从腹股沟区域分离脂肪组织并培养ADSCs。将ADSCs（1 - 3×10⁶）与去细胞胶原蛋白混合后，立即使用23号针头的注射器注入每只动物的右侧声带。作为对照，将去细胞胶原蛋白注入同一只犬的左侧声带。通过形态学和组织学评估来衡量预防声带瘢痕形成和萎缩的效果。在8周时，大多数犬的ADSCs注射侧和对照侧在肉芽肿和萎缩性变化方面存在差异。在24周的随访中，这种差异仍然存在。在组织病理学检查中，初次治疗8周后，在ADSCs注射的声带中观察到大量用荧光染料标记的细胞。本研究证明了ADSCs在受损声带再生中的多能性。在初次损伤24周后，将ADSCs注入受损声带似乎有助于预防声带瘢痕形成和萎缩。
