Fortin Elodie, Vieil Eric, Mailley Pascal, Szunerits Sabine, Livache Thierry
Groupe Chimie de la Reconnaissance et Etude des Assemblages Biologiques - SprAM (UMR 5819 CEA/CNRS/UJF), DRFMC/SPrAM/CREAB, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
Anal Chem. 2007 May 15;79(10):3741-6. doi: 10.1021/ac061765d. Epub 2007 Apr 6.
Electrochemical oxidation of 2'-deoxyguanosine has been performed on boron-doped diamond (BDD) electrodes, resulting in a strong adsorption of the formed oxidized products onto the BDD surface. The adsorption behavior has been investigated by studying the electrochemical behavior of a redox probe ([IrCl6]3-) using cyclic voltammetry. The most probable situations are the formation of (A) an insulating adsorbed film resulting in a partially blocked electrode behavior, (B) a porous film, or (C) an overall conductive film. Different parameters such as the standard rate constant, the charge-transfer coefficient, the electrode/adsorbed products/solution interface resistance, and the formal potential of the redox couple were determined. Through comparison of theoretical current-potential curves obtained by analytical calculations with experimental cyclic voltammograms, we found that the oxidized products of 2'-deoxyguanosine form a continuous conductive film on BDD.
已在掺硼金刚石（BDD）电极上对2'-脱氧鸟苷进行了电化学氧化，导致所形成的氧化产物强烈吸附在BDD表面。通过使用循环伏安法研究氧化还原探针（[IrCl6]3-）的电化学行为，对吸附行为进行了研究。最可能的情况是形成（A）导致电极行为部分受阻的绝缘吸附膜，（B）多孔膜，或（C）整体导电膜。确定了不同参数，如标准速率常数、电荷转移系数、电极/吸附产物/溶液界面电阻以及氧化还原对的形式电位。通过将分析计算得到的理论电流-电位曲线与实验循环伏安图进行比较，我们发现2'-脱氧鸟苷的氧化产物在BDD上形成了连续的导电膜。
