Gandini Attardi D, Martini G, Mattoccia E, Tocchini-Valentini G P
Proc Natl Acad Sci U S A. 1976 Feb;73(2):554-8. doi: 10.1073/pnas.73.2.554.
A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.
本文描述了一种从非洲爪蟾第6期卵母细胞中提取的可溶性无细胞提取物。该提取物能将超螺旋的猴病毒40 DNA转变为带切口的环状DNA(有一个单链切口)、完整单位大小的线性分子、较短长度的片段以及各种形式的复杂DNA。