Maimone F, Colonna B, Bazzicalupo P, Oliva B, Nicoletti M, Casalino M
J Bacteriol. 1979 Aug;139(2):369-75. doi: 10.1128/jb.139.2.369-375.1979.
The plasmids from six clinical strains of Salmonella wien have been characterized. All the S. wien strains were found to carry three types of plasmids: an IncFI R-Tc Cm Km Ap (resistance to tetracycline, chloramphenicol, kanamycin, and ampicillin) plasmid, either conjugative or nonconjugative, of large size (90 to 100 megadaltons); an R-Ap Su Sm (resistance to ampicillin, sulfonamide, and streptomycin) plasmid of 9 megadaltons; and a very small (1.4 megadaltons) cryptic plasmid. The characteristics of conjugative R plasmids, recombinant between F'lac pro and the FI nonconjugative plasmid, indicated that regions coding for the donor phenotype were present on this plasmid. The molecular and genetic features of the R plasmids were very close to those described for the R plasmids isolated from S. wien strains of different origin. This fact supported the hypothesis of a clonal distribution of this serotype in Algeria and Europe. The analysis used to identify transposable elements showed the presence of only TnA elements, which were located on both the R-Tc Cm Km Ap and R-Ap Su Sm plasmids. They contained the structural gene for a TEM-type beta-lactamase and had translocation properties analogous to those reported for other TnA's.
对来自六株维也纳沙门氏菌临床菌株的质粒进行了特性分析。发现所有维也纳沙门氏菌菌株都携带三种类型的质粒：一种IncFI R-Tc Cm Km Ap（对四环素、氯霉素、卡那霉素和氨苄青霉素耐药）质粒，大小为90至100兆道尔顿，可接合或不可接合；一种9兆道尔顿的R-Ap Su Sm（对氨苄青霉素、磺胺和链霉素耐药）质粒；以及一种非常小的（１.4兆道尔顿）隐蔽质粒。F'lac pro与FI非接合性质粒之间重组的接合性R质粒的特性表明，编码供体表型的区域存在于该质粒上。这些R质粒的分子和遗传特征与从不同来源的维也纳沙门氏菌菌株中分离出的R质粒所描述的特征非常接近。这一事实支持了该血清型在阿尔及利亚和欧洲呈克隆分布的假说。用于鉴定转座元件的分析表明，仅存在TnA元件，它们位于R-Tc Cm Km Ap和R-Ap Su Sm质粒上。它们含有TEM型β-内酰胺酶的结构基因，并且具有与报道的其他TnA类似的易位特性。
