Burgio G, Lanzoni A, Accinelli G, Dinelli G, Bonetti A, Marotti I, Ramilli F
Dipartimento di Scienze e Tecnologie Agroambientali, Alma Mater Studiorum-Università di Bologna, Viale Fanin 42, 40127 Bologna, Italy.
Bull Entomol Res. 2007 Apr;97(2):211-5. doi: 10.1017/S0007485307004920.
As consequence of the concern about the biosafety of genetically modified plants, biological and ecological studies are considered crucial for environmental risk assessment. Laboratory experiments were carried out in order to evaluate the transfer of the Cry1Ac Bt-toxin from a transgenic Bt-oilseed rape to a non-target pest, Myzus persicae Sulzer. Cry1Ac protein levels in plants and aphids were determined using a double sandwich enzyme-linked immunosorbent assay. Phloem sap from (Bt+) and (Bt-) oilseed rape plants was collected from leaves using a standard method of extraction in an EDTA buffer. Bt-toxin was present in phloem sap, with a mean concentration of 2.7 +/- 1.46 ppb, corresponding to a 24-fold lower level than in oilseed rape leaves. Toxin was also detected in aphid samples, with a mean concentration in the positive samples of 2.0 +/- 0.8 ppb. The evidence that Bt-toxin remains in herbivores, in this case an aphid, could be useful to clarify functional aspects linked to possible consequences of Bt-crops on food chains involving herbivore-natural enemy trophic systems. Further studies are needed in order to improve the knowledge on the functional aspects linked to the transfer of the Cry1Ac Bt-toxin from GM-oilseed rape to aphids and their possible consequence.
由于对转基因植物生物安全性的关注，生物学和生态学研究被认为对环境风险评估至关重要。开展了实验室实验，以评估Cry1Ac Bt毒素从转基因Bt油菜向非靶标害虫桃蚜（Myzus persicae Sulzer）的转移。使用双夹心酶联免疫吸附测定法测定植物和蚜虫中的Cry1Ac蛋白水平。采用在EDTA缓冲液中的标准提取方法，从叶片中收集（Bt+）和（Bt-）油菜植株的韧皮部汁液。韧皮部汁液中存在Bt毒素，平均浓度为2.7±1.46 ppb，比油菜叶片中的水平低24倍。在蚜虫样本中也检测到了毒素，阳性样本中的平均浓度为2.0±0.8 ppb。Bt毒素残留在食草动物（在此为蚜虫）体内的证据，可能有助于阐明与Bt作物对涉及食草动物-天敌营养系统的食物链可能产生的后果相关的功能方面。需要进一步开展研究，以增进对与Cry1Ac Bt毒素从转基因油菜向蚜虫转移及其可能后果相关的功能方面的了解。
