Guo Jingfei, He Kanglai, Bai Shuxiong, Zhang Tiantao, Liu Yunjun, Wang Fuxin, Wang Zhenying
State Key Laboratory for Biology of Plant Diseases and Insect Pests, MOA - CABI Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Transgenic Res. 2016 Dec;25(6):761-772. doi: 10.1007/s11248-016-9968-y. Epub 2016 Jun 25.
Non-lepidopteran pests are exposed to, and may be influenced by, Bt toxins when feeding on Bt maize that express insecticidal Cry proteins derived from Bacillus thuringiensis (Bt). In order to assess the potential effects of transgenic cry1Ie maize on non-lepidopteran pest species and ecological communities, a 2-year field study was conducted to compare the non-lepidopteran pest abundance, diversity and community composition between transgenic cry1Ie maize (Event IE09S034, Bt maize) and its near isoline (Zong 31, non-Bt maize) by whole plant inspections. Results showed that Bt maize had no effects on non-lepidopteran pest abundance and diversity (Shannon-Wiener diversity index, Simpson's diversity index, species richness, and Pielou's index). There was a significant effect of year and sampling time on those indices analyzed. Redundancy analysis indicated maize type, sampling time and year totally explained 20.43 % of the variance in the non-lepidopteran pest community composition, but no association was presented between maize type (Bt maize and non-Bt maize) and the variance. Nonmetric multidimensional scaling analysis showed that sampling time and year, rather than maize type had close relationship with the non-lepidopteran pest community composition. These results corroborated the hypothesis that, at least in the short-term, the transgenic cry1Ie maize had negligible effects on the non-lepidopteran pest abundance, diversity and community composition.
非鳞翅目害虫在取食表达源自苏云金芽孢杆菌（Bt）的杀虫Cry蛋白的Bt玉米时，会接触到Bt毒素，并可能受到其影响。为了评估转基因cry1Ie玉米对非鳞翅目害虫物种和生态群落的潜在影响，开展了一项为期两年的田间研究，通过对整株植物进行检查，比较转基因cry1Ie玉米（事件IE09S034，Bt玉米）与其近等基因系（综31，非Bt玉米）之间非鳞翅目害虫的丰度、多样性和群落组成。结果表明，Bt玉米对非鳞翅目害虫的丰度和多样性（香农-威纳多样性指数、辛普森多样性指数、物种丰富度和皮洛指数）没有影响。年份和采样时间对所分析的这些指数有显著影响。冗余分析表明，玉米类型、采样时间和年份总共解释了非鳞翅目害虫群落组成中20.43%的变异，但玉米类型（Bt玉米和非Bt玉米）与变异之间没有关联。非度量多维尺度分析表明，与非鳞翅目害虫群落组成关系密切的是采样时间和年份，而非玉米类型。这些结果证实了以下假设：至少在短期内，转基因cry1Ie玉米对非鳞翅目害虫的丰度、多样性和群落组成影响可忽略不计。
