Braz Mariana Gobbo, Fávero Salvadori Daisy Maria
Department of Pathology, Faculty of Medicine, UNESP - São Paulo State University, Rubião Junior, 18618-000 Botucatu, SP, Brazil.
Toxicol In Vitro. 2007 Aug;21(5):972-6. doi: 10.1016/j.tiv.2007.02.006. Epub 2007 Feb 27.
The comet assay has been conducted with numerous cell lines to assess in vitro genotoxicity. In order to use the comet assay as part of an in vitro test for evaluating genotoxicity, however, there are cell-specific factors that need to be better understood. In this present study we have evaluated some factors that may impact upon the DNA damage detected in whole blood (WB) cells and lymphocytes (ILs). Experiments were conducted comparing responses of both cells, and investigating the effects of the female hormonal cycle, and oral contraceptive (OC) use on DNA damage detection in the in vitro comet assay, at three sampling time. No significant differences were detected in the basal levels of DNA damage detected in ILs and WB cells from women OC users and non-users and from men. Basal DNA damage in ILs was unaffected by gender and stage of the menstrual cycle or the stage of the treatment schedule. Our results also indicated that the H2O2 induces DNA damage in human lymphocytes independently of gender, low-dose OC use and hormonal fluctuation. However, data showed that in 3rd sampling of menstrual cycle, lymphocytes were more resistant to H2O2-induced DNA damage than those from OC users and men.
彗星试验已在众多细胞系中进行，以评估体外遗传毒性。然而，为了将彗星试验用作评估遗传毒性的体外试验的一部分，有一些细胞特异性因素需要更好地理解。在本研究中，我们评估了一些可能影响全血（WB）细胞和淋巴细胞（ILs）中检测到的DNA损伤的因素。进行了实验，比较两种细胞的反应，并在三个采样时间研究女性激素周期和口服避孕药（OC）的使用对体外彗星试验中DNA损伤检测的影响。在使用OC的女性和未使用OC的女性以及男性的ILs和WB细胞中检测到的DNA损伤基础水平上未发现显著差异。ILs中的基础DNA损伤不受性别、月经周期阶段或治疗时间表阶段的影响。我们的结果还表明，H2O2在不依赖性别、低剂量OC使用和激素波动的情况下诱导人淋巴细胞中的DNA损伤。然而，数据显示，在月经周期的第3次采样中，淋巴细胞比来自OC使用者和男性的淋巴细胞对H2O2诱导的DNA损伤更具抗性。
