Meyerhans A, Vartanian J P, Wain-Hobson S
Laboratoire de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
Nucleic Acids Res. 1992 Feb 11;20(3):521-3. doi: 10.1093/nar/20.3.521.
A method for the amplification of a single DNA strand at low copy number is described. It is a wholly PCR based approach which involves an initial linear amplification of the target using a tagged strand specific primer. This is followed by classical PCR amplification of the progeny using a pair of primers, one specific for the sequence tagged onto the 5' end of the first round primer, the second specific for the target sequence. Given the protocol used the ratio of the two strands in the final amplification product was 50:1.
描述了一种用于低拷贝数单链DNA扩增的方法。这是一种完全基于PCR的方法，该方法涉及使用带标签的链特异性引物对靶标进行初始线性扩增。随后使用一对引物对后代进行经典PCR扩增，其中一个引物对第一轮引物5'端标记的序列具有特异性，另一个引物对靶标序列具有特异性。根据所使用的方案，最终扩增产物中两条链的比例为50:1。
