通过用M13克隆的单链DNA去除互补链对双链DNA PCR产物进行直接测序。 - Suppr

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超能文献

Direct sequencing of double-stranded DNA PCR products via removing the complementary strand with single-stranded DNA of an M13 clone.

作者信息

Gal S, Hohn B

机构信息

Friedrich Miescher-Institute, Basel, Switzerland.

出版信息

Nucleic Acids Res. 1990 Feb 25;18(4):1076. doi: 10.1093/nar/18.4.1076.

DOI:10.1093/nar/18.4.1076

PMID:2315033

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330393/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/426b/330393/0c10f00fbe99/nar00188-0365-a.jpg

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Direct sequencing from low-melt agarose with Sequenase.使用Sequenase从低熔点琼脂糖中进行直接测序。

Nucleic Acids Res. 1989 Jul 25;17(14):5864. doi: 10.1093/nar/17.14.5864.

PCR and DNA sequencing.聚合酶链反应（PCR）和DNA测序。

Biotechniques. 1989 Jul-Aug;7(7):700-8.

Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication.利用聚合酶链反应（PCR）对微量类病毒RNA进行序列分析。快速通讯。

Arch Virol. 1989;106(3-4):335-40. doi: 10.1007/BF01313962.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。

Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.

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