高脂血症血清和外源性脯氨酸浓度对离体兔主动脉胶原合成的影响。

Effects of hyperlipoproteinemic serum and exogenous proline concentration on collagen synthesis by isolated rabbit aortas.

作者信息

Holderbaum D, Ehrhart L A, McCullagh K G

出版信息

Proc Soc Exp Biol Med. 1975 Nov;150(2):363-7. doi: 10.3181/00379727-150-39036.

Abstract

Collagen synthesis was measured in segments of normal rabbit aorta, incubated in vitro, by monitoring the formation of peptidyl-14C-hydroxyproline from [U-14C]-L-proline added to the incubation medium. The effect of hyperlipoproteinemic rabbit serum on the rate of collagen synthesis was compared with the effect of normal rabbit serum. No differences in the rates of synthesis were detected between the two batches of serum, despite a 60-fold difference in serum cholesterol concentration. Increases in free proline concentration in the incubation medium resulted in changes in proline flux between medium and tissue pools of free proline, but medium proline concentration had no effect on the rate of collagen synthesis.

摘要

通过监测添加到孵育培养基中的[U-¹⁴C]-L-脯氨酸形成肽基-¹⁴C-羟脯氨酸的情况，来测定体外孵育的正常兔主动脉段中的胶原蛋白合成。将高脂蛋白血症兔血清对胶原蛋白合成速率的影响与正常兔血清的影响进行比较。尽管两批血清的胆固醇浓度相差60倍，但未检测到合成速率的差异。孵育培养基中游离脯氨酸浓度的增加导致游离脯氨酸在培养基和组织池之间的脯氨酸通量发生变化，但培养基脯氨酸浓度对胶原蛋白合成速率没有影响。