Rootman Dan B, Wankiewicz Ellen, Sharpen Linda, Baxter Stephanie A
Department of Ophthalmology, Hotel Dieu Hospital, Queen's University, Kingston, Canada.
Cornea. 2007 Apr;26(3):270-3. doi: 10.1097/ICO.0b013e31802c9e05.
There are 2 methods of corneal tissue procurement currently in widespread use: in situ extraction of the corneal button directly to preservation media and whole-globe enucleation of eyes with removal of the button to preservation media at a later time. This study evaluates the effects of these 2 procurement procedures on the initial quality of donor corneal tissue.
Slit-lamp examination results and endothelial cell counts were compared for a total of 468 donor corneas harvested at 2 remote locations: one where in situ procurement was practiced and the other that used whole-globe enucleation procedures.
In both univariate and multivariate analysis, in situ corneas were found to have a lower incidence of moderate or severe haze and folds in Descemet membrane. No differences in mean endothelial cell counts were noted between the 2 populations of donated tissue.
In situ procurement of corneal tissue results in higher initial corneal tissue quality than whole-globe procedures.
目前广泛使用的角膜组织获取方法有两种：将角膜植片原位直接提取至保存介质中，以及眼球整体摘除，随后将角膜植片取出并放入保存介质中。本研究评估这两种获取程序对供体角膜组织初始质量的影响。
对在两个不同地点获取的468个供体角膜的裂隙灯检查结果和内皮细胞计数进行比较：一个地点采用原位获取方法，另一个地点采用眼球整体摘除程序。
在单变量和多变量分析中，均发现原位获取的角膜中，Descemet膜出现中度或重度混浊及褶皱的发生率较低。两组捐赠组织的平均内皮细胞计数未发现差异。
与眼球整体摘除程序相比，原位获取角膜组织可获得更高的角膜组织初始质量。
