十八碳烯酸对日本脑炎病毒在诺维科夫肝癌细胞中生长的影响。

The effect of octadecenoic acid on the growth of japanese encephalitis virus in Novikoff hepatoma cells.

作者信息

Steele W, Jenkin H M

出版信息

Proc Soc Exp Biol Med. 1975 Dec;150(3):630-5. doi: 10.3181/00379727-150-39094.

Abstract

The growth characteristics of Japanese encephalitis virus cultivated in Novikoff hepatoma cells grown in shaker culture can be differentially altered by the presence of 6-cis or cis-9-octadecenoic acid in Swim's 67-G medium. The addition of 125 mug of the 6-isomer per ml medium reduced the number of infectious particles produced, whereas the same amount of the 9-isomer enhanced virus production. The virus was found to be more stable in cell-free spent medium than in fresh medium. The presence of 125 mug 6-18:1 per ml in fresh medium resulted in a rapid loss of virus infectivity.

摘要

在摇瓶培养中生长的诺维科夫肝癌细胞中培养的日本脑炎病毒的生长特性，可因斯维姆67 - G培养基中6 - 顺式或顺 - 9 - 十八碳烯酸的存在而发生不同改变。每毫升培养基中添加125微克的6 - 异构体可减少产生的感染性颗粒数量，而相同量的9 - 异构体则可提高病毒产量。发现该病毒在无细胞的用过的培养基中比在新鲜培养基中更稳定。新鲜培养基中每毫升存在125微克6 - 18:1会导致病毒感染力迅速丧失。