将BHK - 21细胞适应摇瓶培养生长并随后用日本脑炎病毒进行攻击。
Adaptation of BHK-21 cells to growth in shaker culture and subsequent challenge by Japanese encephalitis virus.
作者信息
Guskey L E, Jenkin H M
出版信息
Appl Microbiol. 1975 Sep;30(3):433-8. doi: 10.1128/am.30.3.433-438.1975.
Abstract
Baby hamster kidney (BHK-21) cells were adapted to grow in shaker culture using Waymouth medium 752/1 containing 20 mM N-2-hydroxyethyl-piperazine-N'-2'-ethanesulfonic acid buffer and supplemented with 2.5% (vol/vol) calf serum, 0.002% (wt/vol) sodium oleate, and 0.2% fatty acid-free bovine serum albumin (WO2.5). Infectivity of Japanese encephalitis virus grown in the cells adapted to WO2.5 approached 2 x 10(8) plaque-forming units per ml. The culture volume of infected cells was reduced fivefold 12 h after infection. This step resulted in a 10-fold increase in infectivity over that obtained from infected cultures not subjected to volume reduction.
摘要
幼仓鼠肾（BHK - 21）细胞经适应后，可在含有20 mM N - 2 - 羟乙基哌嗪 - N'- 2' - 乙磺酸缓冲液的Waymouth培养基752/1中进行摇瓶培养，并添加2.5%（体积/体积）小牛血清、0.002%（重量/体积）油酸钠和0.2%无脂肪酸牛血清白蛋白（WO2.5）。在适应WO2.5的细胞中培养的日本脑炎病毒的感染性接近每毫升2×10⁸ 蚀斑形成单位。感染后12小时，感染细胞的培养体积减少了五倍。这一步骤使感染性比未进行体积减少的感染培养物提高了10倍。
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