Ozmen Ozlem, Topsakal Senay, Sahinduran Sima, Ozcelik Mahiye
Department of Pathology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey.
Pancreas. 2007 Apr;34(3):354-8. doi: 10.1097/MPA.0b013e31802f082f.
In this study, we investigated clinically, pathologically, and immunohistochemically the effect of insufficient short-acting insulin treatment on streptozotocin (STZ)-induced diabetes mellitus in rats.
Three groups composed of 10 rats each were studied as follows: (1) a group that received only STZ (50 mg/kg) (STZ group); (2) a group that received 50 mg/kg STZ and, after 12 hours, 8 IU of short-acting insulin treatment (STZ + INS group), repeated every night for 5 days; and (3) a control group. Ketonuria and blood glucose levels were examined every day. Blood was obtained from 2 rats from each group, and necropsy was performed every day during the 5-day period.
Hyperglycemia was observed in the STZ and STZ + INS groups 24 hours after, but levels were higher in the STS + INS group than those in the STZ-only group. Histopathology was similar in the STZ and STZ + INS groups, and degeneration was observed in both groups, but immunohistochemistry revealed a more severe reduction in insulin-secreting cells in the STZ + INS group than that in the STZ group. There were no hyperglycemia and histopathological or immunochemical alteration in the control group.
This study showed that insufficient short-acting insulin treatment can increase the diabetogenic effect of STZ in rats.
在本研究中，我们从临床、病理及免疫组化方面研究了短效胰岛素治疗不足对链脲佐菌素（STZ）诱导的大鼠糖尿病的影响。
将大鼠分为三组，每组10只，具体如下：（1）仅接受STZ（50mg/kg）的组（STZ组）；（2）接受50mg/kg STZ且在12小时后接受8IU短效胰岛素治疗的组（STZ + INS组），每晚重复治疗5天；（3）对照组。每天检查尿酮和血糖水平。每组每天从2只大鼠取血，在5天期间每天进行尸检。
STZ组和STZ + INS组在24小时后均出现高血糖，但STZ + INS组的血糖水平高于仅接受STZ的组。STZ组和STZ + INS组的组织病理学相似，两组均观察到变性，但免疫组化显示STZ + INS组胰岛素分泌细胞的减少比STZ组更严重。对照组未出现高血糖及组织病理学或免疫化学改变。
本研究表明，短效胰岛素治疗不足会增加STZ对大鼠的致糖尿病作用。
