实验性诱导糖尿病大鼠角膜的超微结构改变
The ultrastructural alterations in rat corneas with experimentally-induced diabetes mellitus.
作者信息
Take Gulnur, Karabay Gulten, Erdogan Deniz, Duyar Izzet
机构信息
Department of Histology and Embryology, Faculty of Medicine, Gazi University, Besevler 06500, Ankara, Turkey.
出版信息
Saudi Med J. 2006 Nov;27(11):1650-5.
OBJECTIVE
To examine the ultrastructural changes of rat corneas in streptozotocin (STZ) induced diabetes mellitus and the follow-up insulin treatment.
METHODS
Sprague-Dawley type rats was used for experimental procedures during the period from January to April 2003 at Baskent University, Ankara, Turkey. Rats were studied in 4 groups; group 1: controls, group 2: sham controls (single dose IV sodium citrate), group 3: STZ-induced diabetes mellitus (single dose 45 mg/kg STZ intravenously), group 4: diabetes mellitus + insulin treatment (8 U/day).
RESULTS
We observed degenerative changes in the epithelial layer, stromal keratocytes and endothelial cells in diabetic group. In contrast, the corneal layers have revealed positive alterations in the insulin-treated group. The statistical analyses showed significant narrowing in the epithelial layer in the diabetic group (p=0.002), whereas thickening was observed in the epithelial basement membrane and Descemet's membrane (p=0.002).
CONCLUSION
It was determined that diabetes mellitus causes degenerative changes in cornea, which are positively influenced by short-term insulin treatment.
目的
研究链脲佐菌素（STZ）诱导的糖尿病大鼠角膜的超微结构变化以及后续胰岛素治疗的效果。
方法
2003年1月至4月期间，在土耳其安卡拉的巴斯肯特大学，使用斯普拉格-道利大鼠进行实验。大鼠分为4组；第1组：对照组，第2组：假对照组（静脉注射单剂量柠檬酸钠），第3组：STZ诱导的糖尿病组（静脉注射单剂量45 mg/kg STZ），第4组：糖尿病 + 胰岛素治疗组（8 U/天）。
结果
我们观察到糖尿病组的上皮层、基质角膜细胞和内皮细胞出现退行性变化。相比之下，胰岛素治疗组的角膜各层显示出积极的改变。统计分析表明，糖尿病组上皮层显著变窄（p = 0.002），而上皮层基底膜和Descemet膜增厚（p = 0.002）。
结论
已确定糖尿病会导致角膜发生退行性变化，短期胰岛素治疗对其有积极影响。
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