Take Gulnur, Karabay Gulten, Erdogan Deniz, Duyar Izzet
Department of Histology and Embryology, Faculty of Medicine, Gazi University, Besevler 06500, Ankara, Turkey.
Saudi Med J. 2006 Nov;27(11):1650-5.
To examine the ultrastructural changes of rat corneas in streptozotocin (STZ) induced diabetes mellitus and the follow-up insulin treatment.
Sprague-Dawley type rats was used for experimental procedures during the period from January to April 2003 at Baskent University, Ankara, Turkey. Rats were studied in 4 groups; group 1: controls, group 2: sham controls (single dose IV sodium citrate), group 3: STZ-induced diabetes mellitus (single dose 45 mg/kg STZ intravenously), group 4: diabetes mellitus + insulin treatment (8 U/day).
We observed degenerative changes in the epithelial layer, stromal keratocytes and endothelial cells in diabetic group. In contrast, the corneal layers have revealed positive alterations in the insulin-treated group. The statistical analyses showed significant narrowing in the epithelial layer in the diabetic group (p=0.002), whereas thickening was observed in the epithelial basement membrane and Descemet's membrane (p=0.002).
It was determined that diabetes mellitus causes degenerative changes in cornea, which are positively influenced by short-term insulin treatment.
研究链脲佐菌素（STZ）诱导的糖尿病大鼠角膜的超微结构变化以及后续胰岛素治疗的效果。
2003年1月至4月期间，在土耳其安卡拉的巴斯肯特大学，使用斯普拉格-道利大鼠进行实验。大鼠分为4组；第1组：对照组，第2组：假对照组（静脉注射单剂量柠檬酸钠），第3组：STZ诱导的糖尿病组（静脉注射单剂量45 mg/kg STZ），第4组：糖尿病 + 胰岛素治疗组（8 U/天）。
我们观察到糖尿病组的上皮层、基质角膜细胞和内皮细胞出现退行性变化。相比之下，胰岛素治疗组的角膜各层显示出积极的改变。统计分析表明，糖尿病组上皮层显著变窄（p = 0.002），而上皮层基底膜和Descemet膜增厚（p = 0.002）。
已确定糖尿病会导致角膜发生退行性变化，短期胰岛素治疗对其有积极影响。
