Cheng Xuanhong, Irimia Daniel, Dixon Meredith, Ziperstein Joshua C, Demirci Utkan, Zamir Lee, Tompkins Ronald G, Toner Mehmet, Rodriguez William R
Surgical Services and Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
J Acquir Immune Defic Syndr. 2007 Jul 1;45(3):257-61. doi: 10.1097/QAI.0b013e3180500303.
Simple affordable CD4 cell counting is urgently needed to stage and monitor HIV-infected patients in resource-limited settings. To address the limitations of current approaches, we designed a simple, label-free, and cost-effective CD4 cell counting device using microfluidic technology. We previously described the fabrication of a microfluidic system for high-efficiency isolation of pure populations of CD4+ T cells based on cell affinity chromatography operated under controlled flow. Here, we compare the performance of a microfluidic CD4 cell counting device against standard flow cytometry in 49 HIV-positive subjects over a wide range of absolute CD4 cell counts. We observed a close correlation between CD4 cell counts from the microchip device and measurements by flow cytometry, using unprocessed whole blood from HIV-positive adult subjects. Sensitivities for distinguishing clinically relevant thresholds of 200, 350, and 500 cells/microL are 0.86, 0.90, and 0.97, respectively. Specificity is 0.94 or higher at all thresholds. This device can serve as a functional cartridge for fast, accurate, affordable, and simple CD4 cell counting in resource-limited settings.
在资源有限的环境中，迫切需要简单且经济实惠的CD4细胞计数方法来对HIV感染患者进行分期和监测。为解决当前方法的局限性，我们利用微流控技术设计了一种简单、无标记且经济高效的CD4细胞计数设备。我们之前描述过一种微流控系统的制造方法，该系统基于在可控流动下运行的细胞亲和色谱法，用于高效分离纯CD4 + T细胞群体。在此，我们在49名HIV阳性受试者中，针对广泛的绝对CD4细胞计数范围，将微流控CD4细胞计数设备的性能与标准流式细胞术进行了比较。我们观察到，使用HIV阳性成年受试者的未处理全血时，微芯片设备的CD4细胞计数与流式细胞术测量结果之间存在密切相关性。区分临床相关阈值200、350和500个细胞/微升的灵敏度分别为0.86、0.90和0.97。在所有阈值下，特异性均为0.94或更高。该设备可作为一种功能性试剂盒，用于在资源有限的环境中进行快速、准确、经济且简单的CD4细胞计数。
