Zhang Li-Hua, Yan Dong-Sheng, Zhou Xiang-Tian, Lü Fan, Xie Ruo-Zhong, Wu Guang-Bo, Hu Dan-Ning, Fu Xiao-Ying, Qu Jia
School of Optometry and Ophthalmology, Wenzhou Medical College, Zhejiang 325003, China.
Zhonghua Yan Ke Za Zhi. 2006 Dec;42(12):1109-12.
The aim of this study was to identify the presence of muscarinic acetylcholine receptors-I (M1 receptor) in human retinal pigment epithelium (RPE) in order to determine the role of M1 receptor in the maintenance of function of RPE and its role in the occurrence and development of myopia.
The 3rd-5th passages of RPE cells established in our laboratory were used in the present study. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA expression of M1 receptor in cultured RPE. Immunocytochemistry was used to detect M1 receptor protein in the RPE cells.
Cultured RPE demonstrated mRNA expression of M1 receptor in RT-PCR. Protein of M1 receptor was presented in the RPE under immunocytochemistry.
This study demonstrated the presence of M1 receptor in human RPE at both mRNA and protein levels. M1 receptor plays an important role in the maintenance of function of RPE. Injection of M1 receptor antagonist into the vitreous can delay the occurrence and inhibit the development of myopia, which is possibly related to the inhibition of RPE cells function.
本研究旨在确定毒蕈碱型乙酰胆碱受体-I（M1受体）在人视网膜色素上皮（RPE）中的存在情况，以确定M1受体在维持RPE功能中的作用及其在近视发生发展中的作用。
本研究使用了在我们实验室建立的第3-5代RPE细胞。采用逆转录聚合酶链反应（RT-PCR）检测培养的RPE中M1受体的mRNA表达。采用免疫细胞化学检测RPE细胞中的M1受体蛋白。
在RT-PCR中，培养的RPE显示出M1受体的mRNA表达。在免疫细胞化学下，RPE中呈现出M1受体蛋白。
本研究证明在人RPE中，M1受体在mRNA和蛋白水平均有存在。M1受体在维持RPE功能中起重要作用。向玻璃体注射M1受体拮抗剂可延缓近视的发生并抑制其发展，这可能与抑制RPE细胞功能有关。
