Dong Feng, An Jian-hong, Ren Yue-ping, Yan Dong-sheng, Zhou Xiang-tian, Lü Fan, Hu Dan-ning, Chen Jiang-fan, Qu Jia
School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou 325003, China.
Zhonghua Yan Ke Za Zhi. 2007 Dec;43(12):1110-3.
The aim of this study was to identify the presence of dopamine receptor D2 and adenosine receptor A2A in human retinal pigment epithelium (RPE) in order to determine whether the RPE is a potential site of action for these two receptors.
RPE Cells were isolated and cultured. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry were performed to detect the expression of D2 and A2A receptors in the RPE.
RT-PCR analysis showed that mRNA of D2 and A2A receptors were expressed in the RPE. D2 and A2A receptors were distributed in the RPE by immunocytochemistry.
This study demonstrated the presence of D2 and A2A receptors in human RPE at both mRNA and protein levels. D2 and A2A receptors may play an important role in the maintenance of the function of human retinal pigment epithelium. Furthermore, their cooperation appeared to be important in the development of axial myopia and retinal and choroidal neovascularization.
本研究旨在确定人视网膜色素上皮（RPE）中多巴胺受体D2和腺苷受体A2A的存在情况，以确定RPE是否是这两种受体的潜在作用位点。
分离并培养RPE细胞。进行逆转录-聚合酶链反应（RT-PCR）和免疫细胞化学检测RPE中D2和A2A受体的表达。
RT-PCR分析显示RPE中表达D2和A2A受体的mRNA。免疫细胞化学显示D2和A2A受体分布于RPE中。
本研究在mRNA和蛋白质水平均证实了人RPE中存在D2和A2A受体。D2和A2A受体可能在维持人视网膜色素上皮功能中发挥重要作用。此外，它们的协同作用在轴性近视以及视网膜和脉络膜新生血管形成过程中似乎也很重要。
