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种间DNA微阵列分析确定WblA是链霉菌中抗生素生物合成的多效性下调因子。

Interspecies DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces.

作者信息

Kang Seung-Hoon, Huang Jianqiang, Lee Han-Na, Hur Yoon-Ah, Cohen Stanley N, Kim Eung-Soo

机构信息

Department of Biological Engineering, Inha University, Incheon, Korea.

出版信息

J Bacteriol. 2007 Jun;189(11):4315-9. doi: 10.1128/JB.01789-06. Epub 2007 Apr 6.

DOI:10.1128/JB.01789-06
PMID:17416669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1913423/
Abstract

Using Streptomyces coelicolor microarrays to discover regulators of gene expression in other Streptomyces species, we identified wblA, a whiB-like gene encoding a putative transcription factor, as a down-regulator of doxorubicin biosynthesis in Streptomyces peucetius. Further analysis revealed that wblA functions pleiotropically to control antibiotic production and morphological differentiation in streptomycetes. Our results reveal a novel biological role for wblA and show the utility of interspecies microarray analysis for the investigation of streptomycete gene expression.

摘要

利用天蓝色链霉菌微阵列来发现其他链霉菌物种中基因表达的调控因子，我们鉴定出wblA，一个编码假定转录因子的whiB样基因，它是产紫链霉菌中阿霉素生物合成的负调控因子。进一步分析表明，wblA具有多效性功能，可控制链霉菌中的抗生素生产和形态分化。我们的结果揭示了wblA的一种新的生物学作用，并展示了种间微阵列分析在研究链霉菌基因表达方面的实用性。

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本文引用的文献

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Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor.天蓝色链霉菌不同抗生素生物合成途径之间的交叉调控。
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Evidence that the Streptomyces developmental protein WhiD, a member of the WhiB family, binds a [4Fe-4S] cluster.链霉菌发育蛋白WhiD（WhiB家族成员）结合[4Fe-4S]簇的证据。
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