Nemets E A, Sevastianov V I
Biomaterials Center, Institute of Transplantology and Artificial Organs, Moscow, U.S.S.R.
Artif Organs. 1991 Oct;15(5):381-5. doi: 10.1111/j.1525-1594.1991.tb00747.x.
The influence of the method of heparin (HEP) immobilization on human serum albumin (HSA), fibrinogen (FG), and antithrombin III (AT-III) adsorption, platelet adhesion, and activation on the surface of polyvinylchloride, polyurethane Vitur, and a copolymer of styrene and divinylbenzene was measured. The negative correlation between the degree of irreversibility of plasma protein adsorption and the amount of adsorbed AT-III for HEP, immobilized onto the polymer surface passivated with HSA, FG, and plasma was found. The same negative correlation was observed between the amount of AT-III adsorbed on these systems and the number of adhered platelets. Schemes of the interaction of surface bound-HEP with AT-III, including the influence of an irreversibly adsorbed protein layer and adhered platelets, have been proposed. The essential role of AT-III in heparinized biomaterials/platelet interaction has been shown. A new method of combined immobilization of HEP and platelet adhesion inhibitor has been elaborated on.
测定了肝素（HEP）固定方法对人血清白蛋白（HSA）、纤维蛋白原（FG）和抗凝血酶III（AT-III）在聚氯乙烯、聚氨酯Vitur以及苯乙烯与二乙烯基苯共聚物表面的吸附、血小板黏附和活化的影响。发现对于固定在经HSA、FG和血浆钝化的聚合物表面的HEP，血浆蛋白吸附的不可逆程度与吸附的AT-III量之间存在负相关。在这些体系上吸附的AT-III量与黏附血小板的数量之间也观察到相同的负相关。提出了表面结合的HEP与AT-III相互作用的方案，包括不可逆吸附蛋白层和黏附血小板的影响。已证明AT-III在肝素化生物材料/血小板相互作用中的重要作用。阐述了一种联合固定HEP和血小板黏附抑制剂的新方法。
