Nemets E A, Sevastíanov V I
Med Tekh. 1994 Mar-Apr(2):18-22.
The interaction of heparinized polymer materials with human plasma proteins (serum albumin (SA), fibrinogen (F), antithrombin III (AT-III)) and platelets was studied. The level of SA and F adsorption irreversibility was demonstrated to depend on the mode of heparin immobilization. There was a negative correlation between the parameters of protein adsorption and the level of adsorbed AT-III. The relationship of the count of platelets adhered to the surface of heparinized polymer materials, to the time of contact was shown to be extreme. There was a correlation between the count of adhesive platelets and the parameters of plasma protein and AT-III adsorption. Based on experimental evidence, a scheme was proposed, which describes the impact of protein adsorption processes on the anticoagulative activity of surface-bound heparin, It is concluded that AT-III plays a particular role as the major component of the adsorption layer that is responsible for the nature of interactions of polymer materials with blood at the protein and cellular levels.
研究了肝素化聚合物材料与人血浆蛋白（血清白蛋白（SA）、纤维蛋白原（F）、抗凝血酶III（AT-III））及血小板之间的相互作用。结果表明，SA和F吸附不可逆程度取决于肝素固定化方式。蛋白质吸附参数与吸附的AT-III水平呈负相关。肝素化聚合物材料表面黏附的血小板数量与接触时间的关系呈极值状态。黏附血小板数量与血浆蛋白及AT-III吸附参数之间存在相关性。基于实验证据，提出了一个方案，该方案描述了蛋白质吸附过程对表面结合肝素抗凝活性的影响。得出的结论是，AT-III作为吸附层的主要成分发挥着特殊作用，该吸附层决定了聚合物材料在蛋白质和细胞水平上与血液相互作用的性质。
