Blomquist S, Malmros C, Mårtensson L, Thörne J
Department of Anesthesiology, University Hospital, Lund, Sweden.
Artif Organs. 1991 Oct;15(5):397-401. doi: 10.1111/j.1525-1594.1991.tb00750.x.
Sequestration of 111In-labeled neutrophils (polymorphonuclear leukocytes--PMNs) in the lungs during sham dialysis was studied dynamically using a gamma camera. Five animals were pretreated with cobra venom factor, naja naja (CVF), to achieve depletion of the complement system, mainly C5a. Seven animals were studied without pretreatment with CVF. Central hemodynamics and gas exchange were studied as well as neutrophil function using luminescence and aggregation. In the control group there was a significant sequestration of neutrophils in the lungs, which reached a maximum between 15 and 17 min after the start of hemodialysis. The peripheral neutrophil count decreased concomitantly. These changes were virtually absent in the CVF-treated group. PMN aggregation and luminescence were abolished in CVF-treated animals. Both pulmonary artery pressure and pulmonary vascular resistance increased significantly in the control group, whereas in the CVF group these parameters remained unchanged. There were no differences in blood gases, platelet count, or hematocrit between the groups. The results clearly indicate that the activation of neutrophils within minutes after the start of hemodialysis is greatly dependent on C5a activation because it can be abolished by C5a depletion. This activation is accompanied by changes in the pulmonary circulation with increases in pressure and resistance.
使用γ相机动态研究了假透析期间111铟标记的中性粒细胞（多形核白细胞——PMNs）在肺部的滞留情况。对5只动物用眼镜蛇毒因子（眼镜蛇属，CVF）进行预处理，以实现补体系统（主要是C5a）的耗竭。对7只动物未用CVF进行预处理就进行了研究。研究了中心血流动力学和气体交换，以及使用发光和聚集法检测的中性粒细胞功能。在对照组中，肺部有明显的中性粒细胞滞留，在血液透析开始后15至17分钟达到最大值。外周中性粒细胞计数随之下降。在CVF处理组中几乎没有这些变化。在CVF处理的动物中，PMN聚集和发光被消除。对照组中肺动脉压和肺血管阻力均显著增加，而在CVF组中这些参数保持不变。两组之间的血气、血小板计数或血细胞比容没有差异。结果清楚地表明，血液透析开始后几分钟内中性粒细胞的激活很大程度上依赖于C5a的激活，因为它可以通过C5a耗竭而被消除。这种激活伴随着肺循环的变化，压力和阻力增加。
