• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Chlamydomonas reinhardtii CNX1E reconstitutes molybdenum cofactor biosynthesis in Escherichia coli mutants.莱茵衣藻CNX1E在大肠杆菌突变体中重建钼辅因子生物合成。
Eukaryot Cell. 2007 Jun;6(6):1063-7. doi: 10.1128/EC.00072-07. Epub 2007 Apr 6.
2
Characterization of Chlamydomonas 102 and 104 mutants reveals intermolecular complementation in the molybdenum cofactor protein CNX1E.对 Chlamydomonas 102 和 104 突变体的特征分析揭示了钼辅因子蛋白 CNX1E 中的分子间互补作用。
Protist. 2013 Jan;164(1):116-28. doi: 10.1016/j.protis.2012.04.003. Epub 2012 May 10.
3
Characterization of a mutant of Chlamydomonas reinhardtii deficient in the molybdenum cofactor.莱茵衣藻钼辅因子缺陷型突变体的特性分析
Physiol Plant. 2009 Jul;136(3):336-50. doi: 10.1111/j.1399-3054.2009.01221.x. Epub 2009 Feb 12.
4
Functional specialization of Chlamydomonas reinhardtii cytosolic thioredoxin h1 in the response to alkylation-induced DNA damage.莱茵衣藻胞质硫氧还蛋白h1在响应烷基化诱导的DNA损伤中的功能特化
Eukaryot Cell. 2005 Feb;4(2):262-73. doi: 10.1128/EC.4.2.262-273.2005.
5
Quantitation of molybdopterin oxidation product in wild-type and molybdenum cofactor deficient mutants of Chlamydomonas reinhardtii.莱茵衣藻野生型和钼辅因子缺陷型突变体中钼蝶呤氧化产物的定量分析。
Biochim Biophys Acta. 1992 Dec 28;1160(3):269-74. doi: 10.1016/0167-4838(92)90087-t.
6
Mcp1 encodes the molybdenum cofactor carrier protein in Chlamydomonas reinhardtii and participates in protection, binding, and storage functions of the cofactor.Mcp1编码莱茵衣藻中的钼辅因子载体蛋白，并参与该辅因子的保护、结合和储存功能。
J Biol Chem. 2003 Mar 28;278(13):10885-90. doi: 10.1074/jbc.M211320200. Epub 2003 Jan 7.
7
Two adjacent nuclear genes are required for functional complementation of a chloroplast trans-splicing mutant from Chlamydomonas reinhardtii.莱茵衣藻叶绿体反式剪接突变体的功能互补需要两个相邻的核基因。
Plant J. 2005 Sep;43(5):636-48. doi: 10.1111/j.1365-313X.2005.02478.x.
8
Annotation of genes involved in glycerolipid biosynthesis in Chlamydomonas reinhardtii: discovery of the betaine lipid synthase BTA1Cr.莱茵衣藻中甘油脂质生物合成相关基因的注释：甜菜碱脂质合酶BTA1Cr的发现
Eukaryot Cell. 2005 Feb;4(2):242-52. doi: 10.1128/EC.4.2.242-252.2005.
9
Escherichia coli MoeA and MogA. Function in metal incorporation step of molybdenum cofactor biosynthesis.大肠杆菌MoeA和MogA。在钼辅因子生物合成的金属掺入步骤中发挥作用。
J Biol Chem. 2002 Jul 12;277(28):24995-5000. doi: 10.1074/jbc.M203238200. Epub 2002 May 10.
10
The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C.莱茵衣藻的叶绿体DnaJ同源物CDJ1是一个包含HSP70B、CGE1和HSP90C的多分子伴侣复合体的一部分。
Plant Physiol. 2008 Dec;148(4):2070-82. doi: 10.1104/pp.108.127944. Epub 2008 Oct 17.

引用本文的文献

1
Insertional Mutagenesis as a Strategy to Open New Paths in Microalgal Molybdenum and Nitrate Homeostasis.插入诱变作为在微藻钼和硝酸盐稳态中开辟新途径的策略。
Curr Issues Mol Biol. 2025 May 26;47(6):396. doi: 10.3390/cimb47060396.
2
The structural principles underlying molybdenum insertase complex assembly.钼插入酶复合物组装的结构原理。
Protein Sci. 2023 Sep;32(9):e4753. doi: 10.1002/pro.4753.
3
-A Reference Microorganism for Eukaryotic Molybdenum Metabolism.- 真核生物钼代谢的参考微生物。
Microorganisms. 2023 Jun 27;11(7):1671. doi: 10.3390/microorganisms11071671.
4
Function of Molybdenum Insertases.钼插入酶的功能。
Molecules. 2022 Aug 23;27(17):5372. doi: 10.3390/molecules27175372.
5
Insights into the Cnx1E catalyzed MPT-AMP hydrolysis.CNX1E 催化 MPT-AMP 水解的研究进展。
Biosci Rep. 2020 Jan 31;40(1). doi: 10.1042/BSR20191806.
6
From the Eukaryotic Molybdenum Cofactor Biosynthesis to the Moonlighting Enzyme mARC.从真核生物钼辅因子生物合成到多功能酶 mARC。
Molecules. 2018 Dec 11;23(12):3287. doi: 10.3390/molecules23123287.
7
The functional principle of eukaryotic molybdenum insertases.真核生物钼插入酶的功能原理。
Biochem J. 2018 May 24;475(10):1739-1753. doi: 10.1042/BCJ20170935.
8
Understanding nitrate assimilation and its regulation in microalgae.了解微藻中的硝酸盐同化及其调控。
Front Plant Sci. 2015 Oct 26;6:899. doi: 10.3389/fpls.2015.00899. eCollection 2015.
9
The Chlamydomonas reinhardtii molybdenum cofactor enzyme crARC has a Zn-dependent activity and protein partners similar to those of its human homologue.莱茵衣藻钼辅因子酶crARC具有与人类同源物相似的锌依赖性活性和蛋白质伴侣。
Eukaryot Cell. 2011 Oct;10(10):1270-82. doi: 10.1128/EC.05096-11. Epub 2011 Jul 29.
10
Nitrate assimilation in Chlamydomonas.衣藻中的硝酸盐同化作用。
Eukaryot Cell. 2008 Apr;7(4):555-9. doi: 10.1128/EC.00431-07. Epub 2008 Feb 29.

本文引用的文献

1
Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.对最小的自由生活真核生物塔氏扁藻的基因组分析揭示了许多独特特征。
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11647-52. doi: 10.1073/pnas.0604795103. Epub 2006 Jul 25.
2
Molybdenum cofactor biosynthesis and molybdenum enzymes.钼辅因子生物合成与钼酶
Annu Rev Plant Biol. 2006;57:623-47. doi: 10.1146/annurev.arplant.57.032905.105437.
3
The Mechanism of nucleotide-assisted molybdenum insertion into molybdopterin. A novel route toward metal cofactor assembly.核苷酸辅助钼插入钼蝶呤的机制。金属辅因子组装的新途径。
J Biol Chem. 2006 Jul 7;281(27):18343-50. doi: 10.1074/jbc.M601415200. Epub 2006 Apr 24.
4
Functional genomics of the regulation of the nitrate assimilation pathway in Chlamydomonas.衣藻中硝酸盐同化途径调控的功能基因组学
Plant Physiol. 2005 Feb;137(2):522-33. doi: 10.1104/pp.104.050914. Epub 2005 Jan 21.
5
Comparative genomics of the pennate diatom Phaeodactylum tricornutum.羽纹硅藻三角褐指藻的比较基因组学
Plant Physiol. 2005 Feb;137(2):500-13. doi: 10.1104/pp.104.052829. Epub 2005 Jan 21.
6
The Diatom EST Database.硅藻表达序列标签数据库。
Nucleic Acids Res. 2005 Jan 1;33(Database issue):D344-7. doi: 10.1093/nar/gki121.
7
Synthesis of adenylated molybdopterin: an essential step for molybdenum insertion.腺苷化钼蝶呤的合成：钼插入的关键步骤。
J Biol Chem. 2004 Dec 31;279(53):55241-6. doi: 10.1074/jbc.M409862200. Epub 2004 Oct 25.
8
Structure of the molybdopterin-bound Cnx1G domain links molybdenum and copper metabolism.与钼蝶呤结合的Cnx1G结构域的结构将钼和铜代谢联系起来。
Nature. 2004 Aug 12;430(7001):803-6. doi: 10.1038/nature02681.
9
Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D.超小单细胞红藻梅氏嗜热栖热菌10D的基因组序列。
Nature. 2004 Apr 8;428(6983):653-7. doi: 10.1038/nature02398.
10
Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga Bigelowiella natans.横向基因转移与含次生质体的藻类纳氏比吉洛藻中质体靶向蛋白的进化
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7678-83. doi: 10.1073/pnas.1230951100. Epub 2003 May 30.

莱茵衣藻CNX1E在大肠杆菌突变体中重建钼辅因子生物合成。

Chlamydomonas reinhardtii CNX1E reconstitutes molybdenum cofactor biosynthesis in Escherichia coli mutants.

作者信息

Llamas Angel, Tejada-Jimenez Manuel, González-Ballester David, Higuera José Javier, Schwarz Guenter, Galván Aurora, Fernández Emilio

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, 14071 Córdoba, Spain.

出版信息

Eukaryot Cell. 2007 Jun;6(6):1063-7. doi: 10.1128/EC.00072-07. Epub 2007 Apr 6.

DOI:10.1128/EC.00072-07
PMID:17416894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1951514/
Abstract

We have isolated and characterized the Chlamydomonas reinhardtii genes for molybdenum cofactor biosynthesis, namely, CNX1G and CNX1E, and expressed them and their chimeric fusions in Chlamydomonas and Escherichia coli. In all cases, the wild-type phenotype was restored in individual mutants as well as in a CNX1G CNX1E double mutant. Therefore, CrCNX1E is the first eukaryotic protein able to complement an E. coli moeA mutant.

摘要

我们已经分离并鉴定了莱茵衣藻中参与钼辅因子生物合成的基因，即CNX1G和CNX1E，并在衣藻和大肠杆菌中表达了它们及其嵌合融合体。在所有情况下，野生型表型在单个突变体以及CNX1G CNX1E双突变体中均得以恢复。因此，CrCNX1E是第一个能够互补大肠杆菌moeA突变体的真核蛋白。