Wu-Wang C Y, Wang S L, Yao P, Slomiany A, Slomiany B L
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Arch Oral Biol. 1991;36(9):637-40. doi: 10.1016/0003-9969(91)90015-m.
The binding characteristics of the PGE2 receptor were investigated in membrane preparations from these glands. Specific [3H]PGE2 binding was linear as a function of the membrane protein concentration and reached steady state by 40 min of incubation at 37 degrees C under neutral pH. Scatchard analysis of the binding data produced a curvilinear plot with a Kd of 0.18 nM and Bmax of 1.02 fmol/mg protein for the high-affinity binding sites, and a Kd of 181 nM and Bmax of 5.72 pmol/mg protein for the low-affinity binding sites. A competitive displacement study indicated that the receptor was specific for prostaglandins of the E series. The study is the first to demonstrate the presence of the PGE2 receptor in rat submandibular gland and to provide its biochemical features.
在这些腺体的膜制剂中研究了PGE2受体的结合特性。特异性[3H]PGE2结合与膜蛋白浓度呈线性关系，在中性pH条件下于37℃孵育40分钟达到稳态。对结合数据进行Scatchard分析，得到一条曲线，高亲和力结合位点的Kd为0.18 nM，Bmax为1.02 fmol/mg蛋白，低亲和力结合位点的Kd为181 nM，Bmax为5.72 pmol/mg蛋白。竞争性置换研究表明，该受体对E系列前列腺素具有特异性。该研究首次证明大鼠颌下腺中存在PGE2受体，并提供了其生化特性。
