胃黏膜前列腺素E2受体的特征分析

Characterization of gastric mucosal prostaglandin E2 receptor.

Wu-Wang C Y, Wang S L, Slomiany A, Slomiany B L

UMDNJ-NJ Dental School, Research Center, Newark, N.J. 07103-2400.

Int J Biochem. 1990;22(8):915-9. doi: 10.1016/0020-711x(90)90297-g.

Abstract

The binding characteristics of gastric mucosal prostaglandin (PG) E2 (PGE2) receptor were investigated using mucosal cell membranes from rat stomach. The binding was found to be dependent upon PGE2 and membrane protein concentration, the time of incubation and the pH of the mixture, being highest at pH 3.0. 2. Scatchard analysis of the binding data revealed a curvilinear plot with high affinity binding (Kd = 2 nM; Bmax = 0.106 pmol/mg protein) and low affinity binding (Kd = 319 nM; Bmax = 2.262 pmol/mg protein) sites. 3. Competitive displacement study indicated that the receptor was specific for PGs of the E series, as PGF2 alpha and 6-keto-PGF1 alpha failed to displace the PGE2. 4. The study is the first report to provide biochemical parameters of specific PGE receptors in rat gastric mucosa.

摘要

使用大鼠胃黏膜细胞膜研究了胃黏膜前列腺素（PG）E2（PGE2）受体的结合特性。发现结合依赖于PGE2和膜蛋白浓度、孵育时间以及混合物的pH值，在pH 3.0时结合力最高。2. 对结合数据进行Scatchard分析显示出一条曲线，具有高亲和力结合位点（Kd = 2 nM；Bmax = 0.106 pmol/mg蛋白）和低亲和力结合位点（Kd = 319 nM；Bmax = 2.262 pmol/mg蛋白）。3. 竞争性置换研究表明，该受体对E系列PG具有特异性，因为PGF2α和6-酮-PGF1α不能置换PGE2。4. 该研究是首次提供大鼠胃黏膜中特异性PGE受体生化参数的报告。