[中国汉族人群中连接蛋白37基因C1019T多态性与冠状动脉疾病的关联]

[Association of C1019T polymorphism in the connexin 37 gene and coronary artery disease in Chinese Han population].

作者信息

Han Ya-Ling, Xi Su-Ya, Zhang Xiao-Lin, Yan Cheng-Hui, Yang Yong, Kang Jian

机构信息

Department of Cardiology, Shenyang General Hospital, Cardiovascular Research Institute of PLA, Shenyang 110016, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2007 Jan 9;87(2):100-4.

PMID:17418016

Abstract

OBJECTIVE

To investigate the association between the connexin 37 (CX37) C1019T polymorphism and the susceptibility to coronary artery disease (CAD) in northern Han population of China.

METHODS

A total of 514 CAD patients and 400 healthy controls diagnosed by angiography were genotyped by using polymerase chain reaction-restriction fragment length polymorphism and polyacrylamide gel electrophoresis.

RESULTS

The genotype frequencies of CC, TC and TT in the CX37 C1019T polymorphism was 22.37%, 53.31% and 24.32% in CAD patients, 17.75%, 46.50% and 35.75% in the controls respectively (P = 0.0007). The frequency of the CX37 C allele in CAD patients was significantly higher than that of the control group (49.03% vs 41.00%, OR = 1.38, 95% CI = 1.15 - 1.66, P = 0.0006). The frequency of the C allele carriers (CC + TC) was 75.68% in the CAD group and 64.25% in the control group (P = 0.0002). Compared with the TT homozygote, the CAD risk was significantly increased in the carriers of C allele (CC + TC) (OR = 1.73, 95% CI = 1.30 - 2.30). Subsequent stratified analysis revealed that the frequency of C allele was significantly higher in the male CAD patients than in the male controls (49.37% vs 39.60%, OR = 1.49, 95% CI = 1.18 - 1.89, P = 0.0009). The CAD risk was nearly two-fold increased in the carriers of C allele (CC + TC) than in the TT homozygote (95% CI = 1.38 - 2.78). However in the female population, there was no difference in the CAD risk between the carriers of (CC + TC) type and the TT homozygote (P = 0.24).

CONCLUSION

The C allele in the CX37 gene might be associated with the susceptibility to CAD and potentially plays an important role in the manifestation of coronary atherosclerosis among Chinese.

摘要

目的

探讨中国北方汉族人群中连接蛋白37（CX37）基因C1019T多态性与冠状动脉疾病（CAD）易感性之间的关联。

方法

采用聚合酶链反应-限制性片段长度多态性及聚丙烯酰胺凝胶电泳技术，对514例经血管造影确诊的CAD患者和400例健康对照者进行基因分型。

结果

CAD患者中CX37基因C1019T多态性的CC、TC和TT基因型频率分别为22.37%、53.31%和24.32%，对照组分别为17.75%、46.50%和35.75%（P = 0.0007）。CAD患者中CX37基因C等位基因频率显著高于对照组（49.03%对41.00%，OR = 1.38，95%CI = 1.15 - 1.66，P = 0.0006）。CAD组中C等位基因携带者（CC + TC）的频率为75.68%，对照组为64.25%（P = 0.0002）。与TT纯合子相比，C等位基因携带者（CC + TC）的CAD风险显著增加（OR = 1.73，95%CI = 1.30 - 2.30）。随后的分层分析显示，男性CAD患者中C等位基因频率显著高于男性对照组（49.37%对39.60%，OR = 1.49，95%CI = 1.18 - 1.89，P = 0.0009）。C等位基因携带者（CC + TC）的CAD风险比TT纯合子增加近两倍（95%CI = 1.38 - 2.78）。然而在女性人群中，（CC + TC）型携带者与TT纯合子的CAD风险无差异（P = 0.24）。