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在产生IgA1的细胞中鉴定和表征CMP-唾液酸：GalNAc-IgA1α2,6-唾液酸转移酶

Identification and characterization of CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase in IgA1-producing cells.

作者信息

Raska Milan, Moldoveanu Zina, Suzuki Hitoshi, Brown Rhubell, Kulhavy Rose, Andrasi Judit, Hall Stacy, Vu Huong L, Carlsson Fredric, Lindahl Gunnar, Tomana Milan, Julian Bruce A, Wyatt Robert J, Mestecky Jiri, Novak Jan

机构信息

University of Alabama at Birmingham, Birmingham, AL 35294, USA.

出版信息

J Mol Biol. 2007 May 25;369(1):69-78. doi: 10.1016/j.jmb.2007.03.002. Epub 2007 Mar 12.

DOI:10.1016/j.jmb.2007.03.002
PMID:17418236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1995659/
Abstract

Glycosylation defects occur in several human diseases. In IgA nephropathy, IgA1 contains O-glycans that are galactose-deficient and consist mostly of core 1 alpha2,6 sialylated N-acetylgalactosamine, a configuration suspected to prevent beta1,3 galactosylation. We confirmed the same aberrancy in IgA1 secreted by the human DAKIKI B cell line. Biochemical assays indicated CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase activity in this cell line. However, a candidate enzyme, ST6-GalNAcI, was not transcribed in DAKIKI cells, B cells isolated from blood, or Epstein-Barr virus (EBV)-immortalized IgA1-producing cells from the blood of IgAN patients and healthy controls. Instead, ST6-GalNAcII transcription was detected at a high level. Expression of the ST6-GalNAcII gene and activity of the CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase were higher in IgA1-producing cell lines from IgAN patients than in such cells from healthy controls. These data are the first evidence that human cells that lack ST6-GalNAcI can sialylate core 1 GalNAc-Ser/Thr.

摘要

糖基化缺陷在多种人类疾病中都会出现。在IgA肾病中，IgA1含有缺乏半乳糖的O-聚糖，主要由核心1 α2,6唾液酸化的N-乙酰半乳糖胺组成，这种结构被怀疑会阻止β1,3半乳糖基化。我们在人DAKIKI B细胞系分泌的IgA1中证实了同样的异常情况。生化分析表明该细胞系中存在CMP-神经氨酸：GalNAc-IgA1 α2,6-唾液酸转移酶活性。然而，候选酶ST6-GalNAcI在DAKIKI细胞、从血液中分离出的B细胞或来自IgA肾病患者和健康对照者血液的爱泼斯坦-巴尔病毒（EBV）永生化产生IgA1的细胞中均未转录。相反，检测到ST6-GalNAcII的转录水平很高。来自IgA肾病患者的产生IgA1的细胞系中ST6-GalNAcII基因的表达和CMP-神经氨酸：GalNAc-IgA1 α2,6-唾液酸转移酶的活性高于来自健康对照者的此类细胞。这些数据首次证明了缺乏ST6-GalNAcI的人类细胞能够使核心1 GalNAc-Ser/Thr唾液酸化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/e46bc8abf231/nihms23793f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/975f5fb8b92d/nihms23793f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/b03be4828d6b/nihms23793f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/a6d385f730c6/nihms23793f3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/e46bc8abf231/nihms23793f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/975f5fb8b92d/nihms23793f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/b03be4828d6b/nihms23793f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/a6d385f730c6/nihms23793f3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c0/1995659/e46bc8abf231/nihms23793f4.jpg

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1
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2
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J Am Soc Nephrol. 2006 Apr;17(4):1192-9. doi: 10.1681/ASN.2005101115. Epub 2006 Mar 1.
3
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Adv Exp Med Biol. 2024;1445:101-117. doi: 10.1007/978-981-97-0511-5_8.
4
Catalytic Mechanism of Nonglycosidic C-N Bond Formation by the Pseudoglycosyltransferase Enzyme VldE.假糖基转移酶VldE催化非糖苷C-N键形成的机制
ACS Catal. 2023 Oct 20;13(20):13369-13382. doi: 10.1021/acscatal.3c02404. Epub 2023 Oct 3.
5
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6
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4
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6
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7
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8
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