Corry Janet E L, Hedges Alan J, Jarvis Basil
Department of Clinical Veterinary Science, University of Bristol, Langford, BS40 5DU, UK.
Food Microbiol. 2007 Sep;24(6):652-7. doi: 10.1016/j.fm.2006.10.001. Epub 2006 Dec 11.
Three parallel trials were made of EU methods proposed for the microbiological examination of red meat using two analysts in each of seven laboratories within the UK. The methods involved determination of aerobic colony count (ACC) and Enterobacteriaceae colony count (ECC) using simulated methods and a freeze-dried standardised culture preparation. Trial A was based on a simulated swab test, Trial B a simulated meat excision test and Trial C was a reference test on reconstituted inoculum. Statistical analysis (ANOVA) was carried out before and after rejection of outlying data. Expanded uncertainty values (relative standard deviation x2) for repeatability and reproducibility, based on the log10 cfu/ml, on the ACC ranged from +/-2.1% to +/-2.7% and from +/-5.5% to +/-10.5%, respectively, depending upon the test procedure. Similarly for the ECC, expanded uncertainty estimates for repeatability and reproducibility ranged from +/-4.6% to +/-16.9% and from +/-21.6% to +/-23.5%, respectively. The results are discussed in relation to the potential application of the methods.
在英国的七个实验室中，由两名分析人员对欧盟提出的红肉微生物检验方法进行了三项平行试验。这些方法包括使用模拟方法和冻干标准化培养制剂测定需氧菌落计数（ACC）和肠杆菌科菌落计数（ECC）。试验A基于模拟拭子试验，试验B基于模拟肉类切除试验，试验C是对复溶接种物的参考试验。在剔除异常数据前后进行了统计分析（方差分析）。基于log10 cfu/ml，ACC的重复性和再现性的扩展不确定度值（相对标准偏差x2）分别在±2.1%至±2.7%和±5.5%至±10.5%之间，具体取决于测试程序。同样，对于ECC，重复性和再现性的扩展不确定度估计分别在±4.6%至±16.9%和±21.6%至±23.5%之间。结合这些方法的潜在应用对结果进行了讨论。
