Hughes Kenneth, Zachertowska Alicja, Wan Simmy, Li Lily, Klimaszewski Damian, Euloth Michelle, Hatchette Todd F
Microbix Biosystems Inc., 341 Bering Avenue, Toronto, Ontario, Canada.
Vaccine. 2007 May 30;25(22):4456-63. doi: 10.1016/j.vaccine.2007.03.017. Epub 2007 Mar 26.
A yield enhancement technology for use in influenza vaccine manufacturing has been developed to maximize the recovery of influenza virus from allantoic fluid of virus-infected chick embryos; the standard raw material for influenza vaccine. Virus associated with amorphous debris in the allantoic fluid can be dissociated from the debris and recovered, thereby increasing viral yield. Dissociation can be achieved by subjecting the virus-debris complex to conditions of increased ionic strength at defined pH. Multifold increases in viral yield per ml of allantoic fluid were observed. The degree of yield enhancement is strain-specific, however, increases were observed in all type A and type B influenza strains tested. The heightened influenza virus recoveries can facilitate rapid vaccine manufacture, with increased numbers of doses produced, and may become essential at a time of influenza pandemic.
已开发出一种用于流感疫苗生产的产量提高技术，以最大限度地从病毒感染鸡胚的尿囊液（流感疫苗的标准原料）中回收流感病毒。尿囊液中与无定形碎片相关的病毒可与碎片解离并回收，从而提高病毒产量。通过使病毒-碎片复合物在特定pH值下处于离子强度增加的条件下可实现解离。观察到每毫升尿囊液的病毒产量有几倍的增加。产量提高的程度是菌株特异性的，然而，在所测试的所有甲型和乙型流感菌株中均观察到产量增加。提高的流感病毒回收率可促进疫苗的快速生产，增加生产的剂量数量，并且在流感大流行时可能变得至关重要。
