Katushkina N V, Kolikov V M, Molodkin V M, Vostriukhina O A, Vovk M P
Vopr Virusol. 1985 Nov-Dec;30(6):675-9.
Virus-containing allantoic fluid (VCAF) serves as raw material for influenza virus manufacture in the USSR. VCAF composition varies greatly from batch to batch. The causes of instability of its composition were studied. The main high molecular admixtures determining VCAF instability were found to include proteins and lipids getting into VCAF with amniotic fluid, blood, and yolk of the embryo when VCAF is collected. Physicochemical characteristics (pH, protein and lipid concentrations, protein composition) for VCAF control at pooling are proposed.
含病毒尿囊液（VCAF）在苏联用作流感病毒生产的原料。不同批次的VCAF成分差异很大。对其成分不稳定的原因进行了研究。发现决定VCAF不稳定的主要高分子杂质包括在收集VCAF时随羊水、血液和胚胎卵黄进入VCAF的蛋白质和脂质。提出了在合并时控制VCAF的物理化学特性（pH值、蛋白质和脂质浓度、蛋白质组成）。
