Ohshima Y, Ko H L, Beuth J, Burrichter H, Oette K, Pulverer G
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
Zentralbl Bakteriol. 1991 Aug;275(3):374-81. doi: 10.1016/s0934-8840(11)80303-8.
The goal of the present study was to evaluate the influence of staphylococcal lipoteichoic acid (LTA) on the activation of mononuclear immune cells. A murine tumor necrosis-like factor (TNF-like) was induced in the sera of CD-1 mice which had been primed with heat/formalin-inactivated Propionibacterium avidum KP-40 and subsequently exposed to LTA extracted from Staphylococcus saprophyticus strain S 1. Monoclonal antibody against murine TNF (anti-TNF) significantly inhibited the cytostatic activity of mice sera against transformed L-929 cells. Freshly isolated lymphocytes did not display interleukin 2 (Il-2) receptors, but receptors were expressed on Con A incubated cells and in significantly higher numbers after coexposure to staphylococcal LTA in vitro. Since the induction of TNF (macrophages) and Il-2 receptors (lymphocytes) represent stimulation of the mononuclear immune system, staphylococcal LTA may be considered to be an immunomodifier.
本研究的目的是评估葡萄球菌脂磷壁酸（LTA）对单核免疫细胞激活的影响。在经热/甲醛灭活的嗜酸丙酸杆菌KP-40致敏并随后暴露于从腐生葡萄球菌菌株S 1提取的LTA的CD-1小鼠血清中诱导出一种鼠肿瘤坏死样因子（TNF样）。抗鼠TNF单克隆抗体（抗TNF）显著抑制小鼠血清对转化的L-929细胞的细胞抑制活性。新鲜分离的淋巴细胞不显示白细胞介素2（Il-2）受体，但在体外经伴刀豆球蛋白A孵育的细胞上表达受体，并且在共同暴露于葡萄球菌LTA后受体数量显著增加。由于TNF（巨噬细胞）和Il-2受体（淋巴细胞）的诱导代表单核免疫系统的刺激，葡萄球菌LTA可被认为是一种免疫调节剂。
