Lu Yuangang, Wu Jinjin, Lei Xia
Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing, 400042, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 Mar;21(3):298-301.
To search the most suitable concentration of calcium in the medium for the basement membrane reconstruction in tissue engineering skin in vitro.
Composite chitosan tissue engineering skin was prepared according to previous studies. Four groups were included according to the concentration of calcium (1.00, 1.45, 1.65 and 1.95 mmol/L respectively). After 7 days and 15 days of culture, the histological manifestation of basement membrane in tissue engineering skin was observed by hematoxylin & eosin staining and PAS staining, and collagen IV of basement membrane was detected immunohistochemically at the dermal-epidermal junction.
This tissue engineering skin shared some histological features of normal skin, including a well-differentiated stratified epidermis and a dense dermis. The epithelium in the group of 1.95 mmol/L. Llcium differentiated better than those in other groups. PAS staining showing a regularly red dying strap domain at the dermal-epidermal junction. Collagen IV was positively stained immunohistochemically at the dermal-epidermal junction in the tissue engineering skin.
The above results suggest that the medium with 1.95 mmol/L calcium should be suitable for the growth of composite chitosan tissue engineering skin and the reconstruction of basement membrane.
寻找体外组织工程皮肤基底膜重建中培养基最适宜的钙浓度。
按照先前研究制备复合壳聚糖组织工程皮肤。根据钙浓度分为四组（分别为1.00、1.45、1.65和1.95 mmol/L）。培养7天和15天后，采用苏木精-伊红染色和PAS染色观察组织工程皮肤基底膜的组织学表现，并在真皮-表皮交界处免疫组化检测基底膜的IV型胶原。
这种组织工程皮肤具有一些正常皮肤的组织学特征，包括分化良好的复层表皮和致密的真皮。1.95 mmol/L钙组的上皮细胞分化优于其他组。PAS染色显示真皮-表皮交界处有规则的红色染色带区域。组织工程皮肤的真皮-表皮交界处IV型胶原免疫组化呈阳性染色。
上述结果表明，含1.95 mmol/L钙的培养基应适合复合壳聚糖组织工程皮肤的生长和基底膜的重建。
