Lin Zhenwu, Floros Joanna
Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA.
Physiol Genomics. 2002 Dec 3;11(3):235-43. doi: 10.1152/physiolgenomics.00061.2002.
Random allele expression has recently been observed for several genes including interleukins and genes of the lymphoid system. We studied the hypothesis that the surfactant protein D (SP-D) gene, an innate host defense molecule, exhibits random allele expression in a tissue-specific manner. SP-D gene expression is tissue specific in the 14 tissues studied. Study of SP-D allelic expression in several tissues revealed a balanced biallelic (BB) in lung, and, in several extrapulmonary tissues, a heterogeneous pattern: BB, imbalanced biallelic (IB), and monoallelic (MO). The results from 103 heterozygous rats showed an expression profile in large intestine of BB (22%), IB (58%), and MO (20%). Among eight families, the percent of BB in siblings varied from 0 to 41%, MO from 0 to 33%, and IB from 49 to 83%. The parent-of-origin does not play a role in SP-D allele-specific expression. However, acquired epigenetic factors, family background, or other factors may contribute to the overall pattern of expression.
最近在包括白细胞介素和淋巴系统基因在内的多个基因中观察到随机等位基因表达。我们研究了以下假设：表面活性蛋白D（SP-D）基因作为一种先天性宿主防御分子，以组织特异性方式表现出随机等位基因表达。在所研究的14种组织中，SP-D基因表达具有组织特异性。对几种组织中SP-D等位基因表达的研究显示，肺中存在平衡双等位基因（BB），而在几种肺外组织中，存在异质模式：BB、不平衡双等位基因（IB）和单等位基因（MO）。103只杂合大鼠的结果显示，大肠中的表达谱为BB（22%）、IB（58%）和MO（20%）。在八个家族中，同胞中BB的百分比从0到41%不等，MO从0到33%不等，IB从49到83%不等。亲本来源在SP-D等位基因特异性表达中不起作用。然而，获得性表观遗传因素、家族背景或其他因素可能有助于整体表达模式。
