Chambers Eric W, Meece Jennifer K, McGowan James A, Lovin Diane D, Hemme Ryan R, Chadee Dave D, McAbee Kevin, Brown Susan E, Knudson Dennis L, Severson David W
Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
J Hered. 2007 May-Jun;98(3):202-10. doi: 10.1093/jhered/esm015. Epub 2007 Apr 9.
Microsatellites have proved to be very useful as genetic markers, as they seem to be ubiquitous and randomly distributed throughout most eukaryote genomes. However, our laboratories and others have determined that this paradigm does not necessarily apply to the yellow fever mosquito Aedes aegypti. We report the isolation and identification of microsatellite sequences from multiple genomic libraries for A. aegypti. We identified 6 single-copy simple microsatellites from 3 plasmid libraries enriched for (GA)(n), (AAT)(n), and (TAGA)(n) motifs from A. aegypti. In addition, we identified 5 single-copy microsatellites from an A. aegypti cosmid library. Genetic map positions were determined for 8 microsatellite loci. These markers greatly increase the number of microsatellite markers available for A. aegypti and provide additional tools for studying genetic variability of mosquito populations. Additionally, most A. aegypti microsatellites are closely associated with repetitive elements that likely accounts for the limited success in developing an extensive panel of microsatellite marker loci.
微卫星已被证明作为遗传标记非常有用，因为它们似乎普遍存在且随机分布于大多数真核生物基因组中。然而，我们实验室及其他一些实验室已确定，这种模式不一定适用于黄热病蚊子埃及伊蚊。我们报告了从埃及伊蚊的多个基因组文库中分离和鉴定微卫星序列的情况。我们从3个富含(GA)(n)、(AAT)(n)和(TAGA)(n)基序的质粒文库中鉴定出6个单拷贝简单微卫星。此外，我们从一个埃及伊蚊粘粒文库中鉴定出5个单拷贝微卫星。确定了8个微卫星位点的遗传图谱位置。这些标记极大地增加了可用于埃及伊蚊的微卫星标记数量，并为研究蚊子种群的遗传变异性提供了更多工具。此外，大多数埃及伊蚊微卫星与重复元件密切相关，这可能是开发广泛的微卫星标记位点面板取得有限成功的原因。
