克罗恩病和溃疡性结肠炎中不变自然杀伤T细胞的缺陷。
Deficiency of invariant NK T cells in Crohn's disease and ulcerative colitis.
作者信息
Grose Randall H, Thompson Fiona M, Baxter Alan G, Pellicci Daniel G, Cummins Adrian G
机构信息
Basil Hetzel Institute for Medical Research and Department of Medicine, University of Adelaide, Adelaide, Australia.
出版信息
Dig Dis Sci. 2007 Jun;52(6):1415-22. doi: 10.1007/s10620-006-9261-7. Epub 2007 Apr 10.
The aim of this study was to investigate whether immunoregulatory invariant NK T cells are deficient in Crohn's disease or ulcerative colitis. Blood was collected for flow cytometry from 106 Crohn's disease, 91 ulcerative colitis, and 155 control subjects. Invariant NK T cells were assessed by Valpha24 and (alpha-galactosylceramide/CD1d tetramer markers. Intracellular cytokine was measured after in vitro anti-CD3 antibody stimulation. Valpha24+ T cells were quantified in ileocolonic biopsies as mRNA by real-time PCR and by immunofluorescence. Circulating invariant NK T cells were 5.3% of the control levels in Crohn's (P < 0.001) and 7.9% of the control levels in ulcerative colitis (P < 0.001). Interleukin-4 production was impaired in Crohn's disease and ulcerative colitis. Intestinal Valpha24 mRNA expression was 7% in Crohn's disease (P < 0.05) and 9% in ulcerative colitis (P < 0.05). Intestinal Valpha24+ T cells were 23% in Crohn's disease but not reduced in ulcerative colitis. We conclude that invariant NK T cells are deficient in Crohn's disease and in ulcerative colitis.
本研究旨在调查免疫调节性不变自然杀伤T细胞在克罗恩病或溃疡性结肠炎中是否存在缺陷。收集了106例克罗恩病患者、91例溃疡性结肠炎患者和155例对照者的血液用于流式细胞术检测。通过Vα24和（α-半乳糖神经酰胺/CD1d四聚体标记物评估不变自然杀伤T细胞。体外抗CD3抗体刺激后测量细胞内细胞因子。通过实时聚合酶链反应和免疫荧光法对回结肠活检组织中的Vα24 + T细胞进行mRNA定量。克罗恩病患者循环中的不变自然杀伤T细胞为对照水平的5.3%（P < 0.001），溃疡性结肠炎患者为对照水平的7.9%（P < 0.001）。克罗恩病和溃疡性结肠炎患者白细胞介素-4的产生均受损。克罗恩病患者肠道Vα24 mRNA表达为7%（P < 0.05），溃疡性结肠炎患者为9%（P < 0.05）。克罗恩病患者肠道Vα24 + T细胞为23%，而溃疡性结肠炎患者未减少。我们得出结论，不变自然杀伤T细胞在克罗恩病和溃疡性结肠炎中均存在缺陷。
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