Nakao T, Kon K, Ando S, Hirabayashi Y
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Japan.
Biochim Biophys Acta. 1991 Nov 27;1086(3):305-9. doi: 10.1016/0005-2760(91)90174-g.
A N-glycolyneuraminic acid containing trisialoganglioside was isolated from bovine brains ganglioside mixture using Q-Sepharose. Its chemical structure was characterized as IV3NeuAc, II3NeuAc-NeuGc, Gg4Cer by gas-liquid chromatography, a permethylation study, sialidase degradation, TLC/enzyme-immunostaining, fast atom bombardment-mass spectrometry, fluorometric HPLC and proton nuclear magnetic resonance spectroscopy. This was unique in the mixed sialic acid constituents. (formula; see text) This accounted for 0.78% of the gangliosides. The ceramide structure was almost identical with those of major bovine brain ganglioside, as mainly composed of 18:0 fatty acid (90.9%) and d20:0 sphingosine base.
使用Q-琼脂糖从牛脑神经节苷脂混合物中分离出一种含N-羟乙酰神经氨酸的三唾液酸神经节苷脂。通过气液色谱、全甲基化研究、唾液酸酶降解、薄层色谱/酶免疫染色、快原子轰击质谱、荧光高效液相色谱和质子核磁共振光谱对其化学结构进行了表征，确定为IV3NeuAc、II3NeuAc-NeuGc、Gg4Cer。这在混合唾液酸成分中是独特的。（分子式；见正文）其占神经节苷脂的0.78%。神经酰胺结构与主要牛脑神经节苷脂的结构几乎相同，主要由18:0脂肪酸（90.9%）和d20:0鞘氨醇碱基组成。
