Alessandri J M, Guesnet P, Arfi T S, Durand G
Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, INRA-CRJ, Jouy-en-Josas, France.
Biochim Biophys Acta. 1991 Nov 27;1086(3):340-8. doi: 10.1016/0005-2760(91)90179-l.
Alterations of phospholipid fatty acid composition in the renewing intestine were studied in the infant piglet. Newborn piglets were fed from birth to 2 weeks of age a concentrated cow's milk which defined a standard supply of dietary fatty acids. Phospholipids were isolated from the whole mucosa, isolated intestinal cells and purified brush border membranes. Intestinal cells were isolated according to their position along the crypt-villus axis and cell phospholipids were extracted at each step of differentiation. Changes in fatty acid composition of cell phospholipids were related to those of lactase activity in the corresponding cell homogenates. In cell phospholipids, the relative content of linoleic and linoleic acids increased about 2-fold from crypt base to villus tip. Substantial contents of alkenylacyl glycerophospholipids (plasmalogens) were found in crypt cell phospholipids and in purified brush border membrane phosphatidylethanolamine (11 and 14% of alkenyl groups by weight of total fatty acids, respectively). The proportion of alkenylacyl glycerophospholipids decreased as cells ascended the villus column and became more differentiated. The results show that fatty acid compositional changes in differentiating cell phospholipids occurred in the immature intestine (before weaning) and suggest that these alterations might be related to the appearance of specific functions.
在新生仔猪中研究了更新中的肠道中磷脂脂肪酸组成的变化。新生仔猪从出生到2周龄喂食浓缩牛奶，其定义了膳食脂肪酸的标准供应。从整个黏膜、分离的肠细胞和纯化的刷状缘膜中分离磷脂。根据肠细胞沿隐窝-绒毛轴的位置进行分离，并在分化的每个阶段提取细胞磷脂。细胞磷脂脂肪酸组成的变化与相应细胞匀浆中乳糖酶活性的变化相关。在细胞磷脂中，亚油酸和亚麻酸的相对含量从隐窝底部到绒毛顶端增加了约2倍。在隐窝细胞磷脂和纯化的刷状缘膜磷脂酰乙醇胺中发现了大量的烯基酰基甘油磷脂（缩醛磷脂）（分别占总脂肪酸重量的11%和14%的烯基）。随着细胞沿绒毛柱上升并变得更加分化，烯基酰基甘油磷脂的比例降低。结果表明，分化中的细胞磷脂脂肪酸组成变化发生在未成熟肠道（断奶前），并表明这些变化可能与特定功能的出现有关。
