How many EF-Tu molecules participate in aminoacyl-tRNA binding?
作者信息
Bensch K, Pieper U, Ott G, Schirmer N, Sprinzl M, Pingoud A
机构信息
Abteilung Biophysikalische Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover, Germany.
出版信息
Biochimie. 1991 Jul-Aug;73(7-8):1045-50. doi: 10.1016/0300-9084(91)90146-r.
PMID:1742349
Abstract
The stoichiometry of the EF-Tu-GTP-aminoacyl-tRNA complex has been re-determined by a variety of methods, viz gel filtrations, fluorescence titrations, as well as hydrolysis and RNase protection experiments. The results of these experiments clearly demonstrate that one aminoacyl-tRNA interacts with only one EF-Tu-GTP molecule, in agreement with the established view and in contrast to the recently published results by Ehrenberg et al [6].
摘要
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