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通过大规模串联重复事件产生的细菌 tRNA 基因。

The birth of a bacterial tRNA gene by large-scale, tandem duplication events.

机构信息

Department of Evolutionary Theory, Max Planck Institute for Evolutionary Biology, Plön, Germany.

Asia Pacific Center for Theoretical Physics, Pohang, Republic of Korea.

出版信息

Elife. 2020 Oct 30;9:e57947. doi: 10.7554/eLife.57947.

DOI:10.7554/eLife.57947
PMID:33124983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7661048/
Abstract

Organisms differ in the types and numbers of tRNA genes that they carry. While the evolutionary mechanisms behind tRNA gene set evolution have been investigated theoretically and computationally, direct observations of tRNA gene set evolution remain rare. Here, we report the evolution of a tRNA gene set in laboratory populations of the bacterium SBW25. The growth defect caused by deleting the single-copy tRNA gene, , is rapidly compensated by large-scale (45-290 kb) duplications in the chromosome. Each duplication encompasses a second, compensatory tRNA gene () and is associated with a rise in tRNA-Ser(UGA) in the mature tRNA pool. We postulate that tRNA-Ser(CGA) elimination increases the translational demand for tRNA-Ser(UGA), a pressure relieved by increasing copy number. This work demonstrates that tRNA gene sets can evolve through duplication of existing tRNA genes, a phenomenon that may contribute to the presence of multiple, identical tRNA gene copies within genomes.

摘要

生物体在其所携带的 tRNA 基因的类型和数量上存在差异。虽然已经从理论和计算上研究了 tRNA 基因集进化背后的进化机制，但 tRNA 基因集进化的直接观察仍然很少见。在这里，我们报告了细菌 SBW25 实验室群体中 tRNA 基因集的进化。缺失单拷贝 tRNA 基因 导致的生长缺陷很快被染色体的大规模（45-290 kb）复制所补偿。每个复制都包含第二个补偿性 tRNA 基因 ()，并与成熟 tRNA 池中的 tRNA-Ser(UGA)增加有关。我们假设 tRNA-Ser(CGA)的消除增加了 tRNA-Ser(UGA)的翻译需求，通过增加 拷贝数来缓解这种压力。这项工作表明，tRNA 基因集可以通过现有 tRNA 基因的复制而进化，这种现象可能导致基因组中存在多个相同的 tRNA 基因副本。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/0370a002afa3/elife-57947-resp-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/4f4fd22aa56c/elife-57947-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/61dd6e67c033/elife-57947-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/2533e451b4e6/elife-57947-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/4f48d7b3de51/elife-57947-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/9ae87b4bdbdb/elife-57947-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/0bdf8e893dfa/elife-57947-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/16a2e7b739d0/elife-57947-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/e00aefa5177d/elife-57947-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/1d6cd806b10d/elife-57947-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/b11414bc5367/elife-57947-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/0370a002afa3/elife-57947-resp-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/4f4fd22aa56c/elife-57947-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/61dd6e67c033/elife-57947-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/2533e451b4e6/elife-57947-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/4f48d7b3de51/elife-57947-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/9ae87b4bdbdb/elife-57947-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/0bdf8e893dfa/elife-57947-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/16a2e7b739d0/elife-57947-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/e00aefa5177d/elife-57947-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/1d6cd806b10d/elife-57947-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/b11414bc5367/elife-57947-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b6b/7661048/0370a002afa3/elife-57947-resp-fig1.jpg

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