Shatsky I N, Bakin A V, Bogdanov A A, Vasiliev V D
AN Belozersky Laboratory, Moscow State University, USSR.
Biochimie. 1991 Jul-Aug;73(7-8):937-45. doi: 10.1016/0300-9084(91)90135-n.
A working model of the mRNA path through the ribosome is proposed. According to the model, the template goes around the small ribosomal subunit along the region where its 'head' is separated from other parts of the subunit. The 5'-end of the mRNA fragment covered by the ribosome is located near the 3'-terminus of 16S rRNA, whereas the 3'-terminal residues of the fragment are situated on the outer surface of the subunit, opposite its 'side ledge'. When associated with the 50S subunit, the 30S subunit is oriented in such a manner that the decoding center faces the L7/L12 stalk. Implications of the proposed working model of the mRNA topography for the function of the ribosome are discussed.
提出了一个mRNA通过核糖体途径的工作模型。根据该模型，模板沿着其“头部”与小核糖体亚基其他部分分离的区域围绕小核糖体亚基。被核糖体覆盖的mRNA片段的5'端位于16S rRNA的3'端附近，而该片段的3'端残基位于亚基的外表面，与其“侧壁”相对。当与50S亚基结合时，30S亚基的取向使得解码中心面向L7/L12柄。讨论了所提出的mRNA拓扑结构工作模型对核糖体功能的影响。
